Bruno V, Sortino M A, Scapagnini U, Nicoletti F, Canonico P L
Institute of Pharmacology, University of Catania, Italy.
Funct Neurol. 1995 May-Jun;10(3):121-30.
We have investigated in the present study the effect of Mg(2+)-valproate on necrotic degeneration induced by an excitotoxic insult in primary culture of cerebellar neurons, that is an homogeneous population of glutamatergic neurons. Mg(2+)-valproate protected cultures against glutamate-induced neurotoxicity, acting as an indirect N-methyl-D-aspartate (NMDA) receptor antagonist, thus reducing free radical formation and affecting the biochemical parameters (i.e. 45Ca(2+)-influx, cyclic GMP formation, inositol phospholipid hydrolysis and protein kinase C translocation) that undergo modifications following NMDA receptor activation in cerebellar granule cells.
在本研究中，我们调查了丙戊酸镁对小脑神经元原代培养物中由兴奋性毒性损伤诱导的坏死性退变的影响，小脑神经元原代培养物是谷氨酸能神经元的同质群体。丙戊酸镁保护培养物免受谷氨酸诱导的神经毒性，作为间接的N-甲基-D-天冬氨酸（NMDA）受体拮抗剂起作用，从而减少自由基形成并影响在小脑颗粒细胞中NMDA受体激活后发生改变的生化参数（即45Ca(2+)内流、环鸟苷酸形成、肌醇磷脂水解和蛋白激酶C易位）。
