Shivanna N, Ramesh S R
Department of Studies in Zoology, University of Mysore, India.
Indian J Exp Biol. 1995 Sep;33(9):668-72.
Quantitative and qualitative analysis of accessory gland proteins in four species of D. immigrans group has been made. Quantitative estimation of these proteins during development of the adult male revealed that there is a gradual increase in the quantity of secretions up to 7 days. The secretions constitute 72, 70, 50 and 54.2% in D.n. nasuta, D.s. neonasuta, D. rubida and D. pararubida respectively. Mating experiments showed that more than 1/3 of this stored secretory protein is transferred into the female reproductive tract during first mating. SDS-PAGE analysis of the accessory gland secretions revealed remarkable simplicity in their patterns, including the absence of age dependent alterations. In all the 4 species analysed, only the low mol. wt. fractions are not glycosylated. It was further observed that the extent of similarity between D.n. nasuta and D.s. neonasuta is greater than between D. rubida and D. pararubida.
对伊蚊属迁入组的四个物种的附腺蛋白进行了定量和定性分析。对成年雄性发育过程中这些蛋白质的定量估计表明，分泌物的量在7天内逐渐增加。在纳氏伊蚊、新纳氏伊蚊、红伊蚊和副红伊蚊中，分泌物分别占72%、70%、50%和54.2%。交配实验表明，在首次交配期间，超过1/3的这种储存分泌蛋白会转移到雌性生殖道中。对附腺分泌物的SDS-PAGE分析显示其模式非常简单，包括不存在年龄依赖性变化。在所有分析的4个物种中，只有低分子量部分没有糖基化。进一步观察到，纳氏伊蚊和新纳氏伊蚊之间的相似程度大于红伊蚊和副红伊蚊之间的相似程度。
