Ueno S, Takeda K, Noguchi S, Izumi F, Kawamura M
Department of Biology, University of Occupational and Environmental Health, Kitakyushu, Japan.
J Membr Biol. 1995 Nov;148(1):51-6. doi: 10.1007/BF00234155.
The cRNA for Torpedo californica Na+/K(+)-ATPase beta-subunit (cRNA beta) was injected into Xenopus oocytes alone or with the cRNA for the Na+/K(+)-ATPase alpha-subunit (cRNA alpha). When cRNA beta was injected alone, the amount of the beta-subunit that accumulated in oocytes increased with increasing amounts of injected cRNA beta. When cRNA beta and cRNA alpha were injected simultaneously, less beta-subunit accumulated than when cRNA beta was injected alone, whereas the Na+/K(+)-ATPase activity increased markedly. The decrease in the accumulation of the beta-subunit was dose-dependent upon the cRNA alpha. The mutant beta-subunit unable to assemble with the alpha-subunit accumulated in oocytes independently of cRNA alpha, suggesting that post-translational control mechanisms may serve to reduce the accumulation of the beta-subunit.
将电鳐（Torpedo californica）Na⁺/K⁺-ATP酶β亚基的cRNA（cRNAβ）单独或与Na⁺/K⁺-ATP酶α亚基的cRNA（cRNAα）一起注射到非洲爪蟾卵母细胞中。当单独注射cRNAβ时，卵母细胞中积累的β亚基量随着注射的cRNAβ量增加而增加。当同时注射cRNAβ和cRNAα时，积累的β亚基比单独注射cRNAβ时少，而Na⁺/K⁺-ATP酶活性显著增加。β亚基积累的减少在剂量上依赖于cRNAα。无法与α亚基组装的突变型β亚基在卵母细胞中独立于cRNAα积累，这表明翻译后控制机制可能有助于减少β亚基的积累。
