Iselin C E, Alm P, Schaad N C, Larsson B, Graber P, Andersson K E
Department of Surgery, Geneva University Hospital, Switzerland.
J Urol. 1996 Feb;155(2):763-7.
To investigate the L-arginine/nitric oxide (NO) pathway in the pig isolated ureter.
Functional inhibitory effects mediated by NO were assessed and correlated with cyclic nucleotide levels. Nitric oxide synthase (NOS) activity was measured by monitoring the conversion of [3H]-arginine to [3H]-citrulline. Immunohistochemical studies were performed.
The NO-donor SIN-1 reduced in a concentration-dependent manner the frequency of contractions, whereas NO completely interrupted the contractile activity. In precontracted strips exposed to SIN-1 or NO, there were 6- and 12-fold increases of the cyclic GMP levels in comparison with control preparations. Activity of NOS was moderate. Overall innervation of the ureter was sparse, and there were few NOS-immunoreactive nerves.
Although few NOS-containing nerves were found, pathways regulating the cyclic GMP levels of pig ureteral smooth muscle were demonstrated. Such pathways may be important targets for drugs producing relaxation of the mammalian ureter.
研究猪离体输尿管中的L-精氨酸/一氧化氮（NO）途径。
评估由NO介导的功能抑制作用，并将其与环核苷酸水平相关联。通过监测[3H]-精氨酸向[3H]-瓜氨酸的转化来测量一氧化氮合酶（NOS）活性。进行免疫组织化学研究。
NO供体SIN-1以浓度依赖性方式降低收缩频率，而NO完全阻断收缩活性。在暴露于SIN-1或NO的预收缩条带中，与对照制剂相比，环鸟苷酸水平增加了6倍和12倍。NOS活性中等。输尿管的整体神经支配稀疏，且几乎没有NOS免疫反应性神经。
尽管发现含NOS的神经很少，但已证明存在调节猪输尿管平滑肌环鸟苷酸水平的途径。这些途径可能是使哺乳动物输尿管松弛的药物的重要靶点。
