Katoh H, Noda N, Hayashi H, Satoh H, Terada H, Ohno R, Yamazaki N
Third Department of Internal Medicine, Hamamatsu University School of Medicine, Japan.
Jpn Heart J. 1995 Sep;36(5):647-56. doi: 10.1536/ihj.36.647.
We measured intracellular NA+ concentration ([Na+]i) of diabetic rat ventricular myocytes using sodium-binding benzofuran isophthalate (SBFI). We used diabetic rats at 8 weeks after the injection of streptozotocin (45 mg/kg i.v.). The level of [Na+]i during the control perfusion was significantly lower in diabetic myocytes than that in normal myocytes (9.2 +/- 0.4 mM v.s. 12.0 +/- 0.3 mM, p < 0.01). After the 40 min perfusion of 1 microM hexamethylene amiloride (HMA), [Na+]i decreased significantly in both groups. However, there was no difference in [Na+]i between the two groups after the perfusion of HMA. It was suggested that the lower [Na+]i in diabetic myocytes could be due to the decreased activity of Na+/H+ exchange system.
我们使用钠结合苯并呋喃异酞酸酯（SBFI）测量了糖尿病大鼠心室肌细胞的细胞内钠离子浓度（[Na⁺]i）。我们使用了在注射链脲佐菌素（45mg/kg静脉注射）8周后的糖尿病大鼠。在对照灌注期间，糖尿病心肌细胞中的[Na⁺]i水平显著低于正常心肌细胞（9.2±0.4mM对12.0±0.3mM，p<0.01）。在灌注1μM六亚甲基阿米洛利（HMA）40分钟后，两组中的[Na⁺]i均显著降低。然而，灌注HMA后两组之间的[Na⁺]i没有差异。提示糖尿病心肌细胞中较低的[Na⁺]i可能是由于Na⁺/H⁺交换系统活性降低所致。
