Morita S, Tsuchiya S, Fujie H, Itano M, Ohashi Y, Minegishi M, Imaizumi M, Endo M, Takano N, Konno T
Department of Pediatric Oncology, Tohoku University, Sendai, Japan.
Leukemia. 1996 Jan;10(1):102-5.
We produced a monoclonal antibody MTK1 which recognized c-kit protein. Using MTK1, 31 leukemia cell lines and 76 leukemia blasts from pediatric patients were analyzed for expression of the c-kit receptor by flow cytometry. The c-kit receptor was detectable on four of four cell lines assigned to the megakaryo/erythromegakaryoblastic lineage and on one of seven cell lines of myeloid lineage. C-kit expression was not seen on any of 20 cell lines of erythroid and lymphoid lineages. Furthermore, c-kit was expressed on 16 of 24 nonlymphoid blasts without platelet surface antigens (67%) and on six of eight non-lymphoid blasts with platelet surface antigens (75%), but was not detectable on 44 lymphoid blasts from pediatric leukemia patients. In these cases CD34 was expressed on 26 of 32 myeloid blasts (81%) and on 27 of 44 lymphoid blasts (61%). The findings indicate a dominant expression of the c-kit receptor on established cell lines assigned to the megakaryo/erythromegakaryoblastic lineage, though a high percentage of leukemic myeloblasts also expressed the c-kit receptor on their surface.
我们制备了一种识别c-kit蛋白的单克隆抗体MTK1。使用MTK1，通过流式细胞术分析了31种白血病细胞系和76例儿科患者的白血病原始细胞中c-kit受体的表达情况。在所检测的4种属于巨核/红巨核母细胞系的细胞系中，均检测到c-kit受体；在7种髓系细胞系中，有1种检测到c-kit受体。在20种红系和淋巴系细胞系中均未检测到c-kit表达。此外，在24例无血小板表面抗原的非淋巴细胞原始细胞中有16例（67%）表达c-kit，在8例有血小板表面抗原的非淋巴细胞原始细胞中有6例（75%）表达c-kit，但在儿科白血病患者的44例淋巴细胞原始细胞中均未检测到c-kit。在这些病例中，32例髓系原始细胞中有26例（81%）表达CD34，44例淋巴细胞原始细胞中有27例（61%）表达CD34。这些结果表明，在归属于巨核/红巨核母细胞系的已建细胞系中，c-kit受体呈优势表达，尽管有较高比例的白血病髓系原始细胞表面也表达c-kit受体。
