Matsumura I, Kanakura Y, Ikeda H, Ishikawa J, Yoshida H, Horikawa Y, Nishiura T, Tahara T, Kato T, Miyazaki H, Matsuzawa Y
Second Department of Internal Medicine, Osaka University Medical School, Japan.
Leukemia. 1996 Jan;10(1):91-4.
Thrombopoietin (TPO) is a recently identified hematopoietic growth factor that is essential for the growth and development of megakaryocytes. We have previously shown that TPO induces proliferation of acute myeloblastic leukemia (AML) cells in vitro. In this study, we have examined the expression of TPO and its receptor c-mpl in a series of AML cases and human leukemia cell lines. The mRNA transcripts of TPO were detectable in 18 of 50 AML cases and in some myeloid leukemia cell lines (HEL, M07E and CMK) by means of reverse transcriptase polymerase chain reaction (RT-PCR). In addition, TPO transcripts were coexpressed with c-mpl transcripts in 10 of 50 AML cases and in HEL, M07E and CMK cells. With regard to the French-American-British (FAB) classification, coexpression OF TPO and c-mpl was observed with high frequency in AML cases of M7-type. Despite the TPO expression in a substantial fraction of leukemia cells, biological activity of TPO was not found in the conditioned medium that was obtained from cultivation of TPO mRNA-positive leukemia cells. These results suggest that TPO may not commonly participate in the abnormal growth of AML cells as an extracellular autocrine growth factor.
血小板生成素（TPO）是一种最近发现的造血生长因子，对巨核细胞的生长和发育至关重要。我们之前已表明，TPO在体外可诱导急性髓性白血病（AML）细胞增殖。在本研究中，我们检测了一系列AML病例及人白血病细胞系中TPO及其受体c-mpl的表达情况。通过逆转录聚合酶链反应（RT-PCR），在50例AML病例中的18例以及一些髓系白血病细胞系（HEL、M07E和CMK）中可检测到TPO的mRNA转录本。此外，在50例AML病例中的10例以及HEL、M07E和CMK细胞中，TPO转录本与c-mpl转录本共同表达。关于法美英（FAB）分类，在M7型AML病例中高频观察到TPO和c-mpl的共同表达。尽管相当一部分白血病细胞中有TPO表达，但从培养TPO mRNA阳性白血病细胞获得的条件培养基中未发现TPO的生物活性。这些结果表明，TPO可能通常不会作为细胞外自分泌生长因子参与AML细胞的异常生长。
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