Adams J L, Czuprynski C J
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, USA.
Microb Pathog. 1995 Jul;19(1):19-29. doi: 10.1006/mpat.1995.0041.
Johne's disease is a chronic enteritis of cattle and other ruminant species that is of worldwide economic importance. The cytokines tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) have been associated with granuloma formation and wasting in other disease syndromes. The potential role of these cytokines in the development and progression of Johne's disease has not been investigated. Using reverse transcriptase polymerase chain reaction (RT-PCR) and specific bovine oligonucleotide cytokine primers and probes for bovine TNF-alpha and IL-6, we examined the ex vivo expression of mRNA for these inflammatory cytokines in whole blood from healthy cattle. Cytokine mRNA levels increased after a brief incubation of bovine whole blood with Mycobacterium paratuberculosis or its lipoarabinomannan (LAM). Muramyl dipeptide (MDP) and Escherichia coli LPS also stimulated TNF-alpha and IL-6 mRNA expression. Several strains of M. paratuberculosis were tested and found to have similar abilities to stimulate TNF-alpha and IL-6 mRNA expression. Several strains of the closely related Mycobacterium avium, and the unrelated saprophyte, Mycobacterium phlei, had somewhat less ability to stimulate TNF-alpha and IL-6 mRNA expression.
副结核病是一种影响牛和其他反刍动物的慢性肠炎，在全球范围内具有重要的经济意义。细胞因子肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）与其他疾病综合征中的肉芽肿形成和消瘦有关。这些细胞因子在副结核病发生和发展中的潜在作用尚未得到研究。我们使用逆转录聚合酶链反应（RT-PCR）以及针对牛TNF-α和IL-6的特异性牛寡核苷酸细胞因子引物和探针，检测了健康牛全血中这些炎性细胞因子mRNA的体外表达。用副结核分枝杆菌或其脂阿拉伯甘露聚糖（LAM）短暂孵育牛全血后，细胞因子mRNA水平升高。胞壁酰二肽（MDP）和大肠杆菌脂多糖（LPS）也刺激TNF-α和IL-6 mRNA表达。对几株副结核分枝杆菌进行了测试，发现它们刺激TNF-α和IL-6 mRNA表达的能力相似。几株密切相关的鸟分枝杆菌以及不相关的腐生菌草分枝杆菌刺激TNF-α和IL-6 mRNA表达的能力略弱。
