Mårtensson S, Brunmark C, Ohlsson L, Bak-Jensen E, Butkowski R, Boketoft A, Wieslander J
Department of Urology, University of Lund, Sweden.
Nephrol Dial Transplant. 1995;10(9):1637-43.
Monoclonal antibodies were used to study the expression of three recently characterized basement membrane components and two carbohydrate antigens in 11 renal-cell carcinomas, using immunohistological and biochemical techniques. The expression of several site-specific kidney antigens in renal-cell carcinoma were studied to determine the origin of the carcinoma and if it is possible further classify this type of carcinoma. Tubulointerstitial nephritis antigen (TIN) and two alpha-chains of type IV collagen, alpha 1 (IV) and alpha 3 (IV) were studied. In addition the expression of carbohydrate antigens Lex and SLex, which also exhibit site-specific distribution were characterized. Lex and SLex antibodies stained the majority of the tumours. TIN was expressed in 9 of 11 tumours, the alpha 1 (IV) chain was present in all 11, and the alpha 3 (IV) chain in two of the 11 tumours. Interestingly, the two alpha 3 (IV)-positive tumours were the same two that were negative for TIN. In normal tissue alpha 3 (IV) is found in distal tubules while TIN is found in proximal tubules. Our results are consistent with earlier observations that the proximal tubule is the origin of most renal-cell carcinomas, but the results also indicate that renal-cell carcinoma may originate from the distal tubule.
采用免疫组织化学和生化技术，运用单克隆抗体研究了11例肾细胞癌中三种最近鉴定出的基底膜成分和两种碳水化合物抗原的表达情况。研究肾细胞癌中几种位点特异性肾抗原的表达，以确定癌的起源以及是否有可能进一步对这种类型的癌进行分类。研究了肾小管间质性肾炎抗原（TIN）以及IV型胶原的两条α链，即α1（IV）和α3（IV）。此外，还对同样呈现位点特异性分布的碳水化合物抗原Lex和SLex的表达进行了鉴定。Lex和SLex抗体对大多数肿瘤进行了染色。11例肿瘤中有9例表达TIN，11例肿瘤均存在α1（IV）链，11例肿瘤中有2例存在α3（IV）链。有趣的是，α3（IV）呈阳性的这两例肿瘤正是TIN呈阴性的那两例。在正常组织中，α3（IV）存在于远端小管，而TIN存在于近端小管。我们的结果与早期观察结果一致，即大多数肾细胞癌起源于近端小管，但结果也表明肾细胞癌可能起源于远端小管。
