Chen X H, Itoh M, Miki T, Ichiyama M, Fujimoto Y, Sun W, Takeuchi Y
Department of Anatomy, Kagawa Medical School, Japan.
Okajimas Folia Anat Jpn. 1995 Aug;72(2-3):69-79. doi: 10.2535/ofaj1936.72.2-3_69.
Serial 30 microns-thick sections through the midbrain tegmentum were stained with cresyl violet. The PL was found to be situated along the medial edge of the lateral lemniscus. The PL consisted of small- (10-15 microns) and medium-sized neurons (25-35 microns), and was the most prominent at the caudal level of the superior colliculus. In order to confirm the existence of the inhibitory paralemniscal-facial pathway, a combined HRP and immunohistochemical technique was use in the rat. This experiment revealed that 10.9% of the total number of GABA immunoreactive PL neurons also labeled with HRP after HRP injection was made in the medial part of the facial nucleus (FN). Electron microscopic observations were carried out on the medial part of the facial nucleus (FN) after kainic acid injection was made into the contralateral PL in the cat. The majority of degenerating PL fibers were ranged from 0.5 to 3.1 microns in diameter and made synaptic contacts with somata, proximal dendrites and dendritic profiles. These fibers, containing either round or pleomorphic vesicles, formed asymmetrical or symmetrical synapses. It was of particular interest in the present study that 40.7% of the total number of degenerating fibers make synaptic contacts with large dendrites more than 3.0 microns in diameter.
将中脑被盖制成连续的30微米厚切片，用甲酚紫染色。发现脑桥旁外侧核位于外侧丘系的内侧边缘。脑桥旁外侧核由小神经元（10 - 15微米）和中型神经元（25 - 35微米）组成，在上丘尾部水平最为明显。为了证实脑桥旁外侧核 - 面神经抑制性通路的存在，在大鼠中使用了结合辣根过氧化物酶（HRP）和免疫组织化学的技术。该实验显示，在面神经核（FN）内侧注射HRP后，脑桥旁外侧核中GABA免疫反应阳性神经元总数的10.9%也被HRP标记。在猫的对侧脑桥旁外侧核注射红藻氨酸后，对面神经核（FN）内侧进行了电子显微镜观察。大多数退变的脑桥旁外侧核纤维直径在0.5至3.1微米之间，并与胞体、近端树突和树突轮廓形成突触联系。这些纤维含有圆形或多形性囊泡，形成不对称或对称突触。在本研究中特别有趣的是，退变纤维总数的40.7%与直径超过3.0微米的大树突形成突触联系。
