Berensztein E, Belgorosky A, de Dávila M R, Rivarola M A
Laboratorio de Investigación, Hospital de Pediatría Garrahan, Buenos Aires, Argentina.
Pediatr Res. 1995 Oct;38(4):592-7. doi: 10.1203/00006450-199510000-00020.
Little is known on the hormonal regulation of the early postnatal phase of Leydig cell activation in the human. Testosterone secretion by prepubertal testicular cells in culture was studied in two different age groups, 0-7-mo-old (group 1) and 16-36-mo-old (group 2) boys. A mixed cell preparation was isolated from testes collected at necropsy and maintained in culture for 6 d. Cells were cultured in serum-free medium in basal conditions and under the stimulation of human (h)LH, hFSH, or recombinant hGH, and the secretion of testosterone was determined on d 6 by RIA. In basal conditions, cells of group 1 secreted more testosterone (median 5.83 pmol/10(6) cells.d, n = 7) than cells of group 2 (median 1.73, n = 5), p < 0.05, reflecting the steroidogenic potential of the testes in vivo. Under hLH stimulation, cells of group 1 responded by increasing testosterone secretion significantly. Surprisingly, hFSH stimulation elicited a similar response in cells of group 1. Because FSH receptors are presumably located in Sertoli cells, it is concluded that these cells secreted a paracrine factor that stimulated testosterone secretion by Leydig cells. On the other hand, recombinant hGH also stimulated the secretion of testosterone by cells of group 1. Recombinant hGH could have interacted with either GH or prolactin receptors of testicular cells. Cells of group 2 did not respond to any stimulus. Because serum levels of LH, FSH, GH, and prolactin are higher during the first months of life than later in childhood, it is possible that the early postnatal activation of the testis is under multiple pituitary hormone influence.
关于人类睾丸间质细胞产后早期激活的激素调节知之甚少。我们研究了两个不同年龄组的培养前青春期睾丸细胞的睾酮分泌情况，即0 - 7个月大（第1组）和16 - 36个月大（第2组）的男孩。从尸检收集的睾丸中分离出混合细胞制备物，并在培养中维持6天。细胞在基础条件下和在人（h）促黄体生成素（LH）、h促卵泡生成素（FSH）或重组h生长激素（GH）刺激下于无血清培养基中培养，在第6天通过放射免疫分析（RIA）测定睾酮分泌。在基础条件下，第1组细胞分泌的睾酮（中位数为5.83 pmol/10⁶个细胞·天，n = 7）比第2组细胞（中位数为1.73，n = 5）多，p < 0.05，这反映了体内睾丸的类固醇生成潜力。在hLH刺激下，第1组细胞通过显著增加睾酮分泌做出反应。令人惊讶的是，hFSH刺激在第1组细胞中引发了类似的反应。由于FSH受体可能位于支持细胞中，因此得出结论，这些细胞分泌了一种旁分泌因子，刺激了间质细胞的睾酮分泌。另一方面，重组hGH也刺激了第1组细胞的睾酮分泌。重组hGH可能与睾丸细胞的GH或催乳素受体相互作用。第2组细胞对任何刺激均无反应。由于出生后最初几个月血清中LH、FSH、GH和催乳素的水平高于儿童期后期，因此产后早期睾丸的激活可能受到多种垂体激素的影响。
