鲁伯H-35大鼠肝癌细胞中金属硫蛋白的诱导

Induction of metallothionein in the Reuber H-35 rat hepatoma cell.

作者信息

Yang M S, Tang M K, Wong R N

机构信息

Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong.

出版信息

Toxicology. 1995 Dec 15;104(1-3):99-104. doi: 10.1016/0300-483x(95)03149-a.

DOI:10.1016/0300-483x(95)03149-a

PMID:8560508

Abstract

The feasibility of using the Reuber H-35 rat hepatoma cell (RH-35 cells) as model for studying metallothionein induction was examined. The RH-35 cells were treated with Cd, a toxic metal which is known to induce metallothionein. The LC50 after a 3-h treatment was 70 microM. The value was significantly higher (P < 0.05) if the cells were pre-treated with a sublethal dose of CdCl2 (5 microM) for 2 days, indicating that pre-treatment with a low dose of Cd can protect against a subsequent higher dose of the same metal. Both the mRNA and the gene product metallothionein can be identified in the cells 2 days after treatment with 5 microM Cd. In addition to Cd, Zn and Cu were also able to induce the expression of metallothionein to various degrees. The results indicate that the MT gene is present in RH-35 cells and is responsive to treatment with various metals. Thus, this cell line can be used as a model to study metallothionein induction.

摘要

研究了使用鲁伯H - 35大鼠肝癌细胞（RH - 35细胞）作为研究金属硫蛋白诱导模型的可行性。用镉处理RH - 35细胞，镉是一种已知能诱导金属硫蛋白的有毒金属。3小时处理后的半数致死浓度（LC50）为70微摩尔。如果细胞先用亚致死剂量的氯化镉（5微摩尔）预处理2天，该值会显著更高（P < 0.05），这表明用低剂量镉预处理可保护细胞免受随后更高剂量相同金属的伤害。在用5微摩尔镉处理细胞2天后，细胞中既能鉴定出金属硫蛋白的信使核糖核酸（mRNA），也能鉴定出其基因产物金属硫蛋白。除了镉，锌和铜也能不同程度地诱导金属硫蛋白的表达。结果表明金属硫蛋白（MT）基因存在于RH - 35细胞中，且对各种金属处理有反应。因此，该细胞系可作为研究金属硫蛋白诱导的模型。