Feitz W F, Steegers E A, Aarnink R G, Arts T, De Vries J D, Van der Wildt B
Department of Urology, University Hospital Nijmegen, The Netherlands.
Urology. 1996 Jan;47(1):118-9. doi: 10.1016/s0090-4295(99)80395-1.
Prenatal ultrasonographic investigations have led to an increasing number of prenatally detected abnormalities, of which a large number involves the urogenital tract. This study was performed to evaluate if endoscopic intra-amniotic access is possible in primates.
In 10 midtrimester rhesus monkeys (Macaca mulatta), endoscopic intrauterine fetoscopy was performed with three access cannulas. Using a Seldinger technique, a vascular access system, and a pediatric laparoscopy set, intra-amniotic inspection was attempted. Fetal growth throughout pregnancy was monitored by ultrasonographic measurements of fetal biometry.
Intrauterine access could successfully be achieved in 10 rhesus monkeys with three cannulas. After partial amniotic fluid exchange, adequate fetoscopy was always possible. Two monkeys aborted on the second and sixth postoperative days. Serial ultrasonographic investigations for fetal biometry showed no disturbance of the intrauterine growth patterns in the remaining 8 monkeys.
We currently conclude that the rhesus monkey model for experimental intrauterine endoscopic surgery may be suitable for study of the developmental abnormalities of the genitourinary tract.
产前超声检查发现的异常情况越来越多，其中大量涉及泌尿生殖道。本研究旨在评估灵长类动物是否可行内镜羊膜腔内穿刺。
对10只妊娠中期的恒河猴（猕猴），使用三个穿刺套管进行内镜宫内胎儿镜检查。采用Seldinger技术、血管穿刺系统和小儿腹腔镜设备，尝试进行羊膜腔内检查。整个孕期通过超声测量胎儿生物指标来监测胎儿生长情况。
10只恒河猴使用三个套管均成功实现宫内穿刺。部分羊水置换后，总能进行充分的胎儿镜检查。两只猴子分别在术后第二天和第六天流产。对其余8只猴子进行的连续超声胎儿生物测量检查显示，其宫内生长模式未受干扰。
我们目前得出结论，恒河猴实验性宫内内镜手术模型可能适用于研究泌尿生殖道发育异常。
