猪霍乱病毒5'末端的特征分析。
Characterization of the hog cholera virus 5' terminus.
作者信息
Katayama K, Kurihara C, Fukushi S, Hoshino F B, Ishikawa K, Nagai H, Ando T, Oya A
机构信息
Basic Research Division, BioMedical Laboratories, Saitama, Japan.
出版信息
Virus Genes. 1995;10(2):185-7. doi: 10.1007/BF01702600.
Hog cholera virus (HoCV) 5' terminus of the ALD and GPE(-) strains were analyzed by using rapid amplification of cDNA end method (5'RACE). An additional nine nucleotides were found at the 5' termini of genomic RNA in the ALD and GPE(-) strains of HoCV. These nine nucleotides were also conserved in BVDV and were suggested to form a hairpin structure at the 5' terminus by computer-assisted analysis. It seems possible that the secondary structure and/or the 5' terminus sequence has a significant role in the HoCV virus genome.
采用cDNA末端快速扩增法（5'RACE）分析了猪霍乱病毒（HoCV）ALD和GPE（-）毒株的5'末端。在HoCV的ALD和GPE（-）毒株的基因组RNA的5'末端发现了另外九个核苷酸。这九个核苷酸在牛病毒性腹泻病毒（BVDV）中也保守，并且通过计算机辅助分析表明在5'末端形成发夹结构。二级结构和/或5'末端序列似乎可能在HoCV病毒基因组中起重要作用。
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