Deng R, Brock K V
Ohio Agricultural Research and Development Center, Food Animal Health Research Program, Wooster 44691.
Nucleic Acids Res. 1993 Apr 25;21(8):1949-57. doi: 10.1093/nar/21.8.1949.
Within the conserved 5' untranslated region (UTR) of the pestivirus genome three highly variable regions were identified. Preceding the polyprotein start codon, multiple cryptic AUG codons and several small open reading frames are characteristic for all the five pestiviruses. Inspection of the context of AUGs revealed that the polyprotein initiation AUG of pestivirus has a weak context for efficient translation initiation. The most favorable context was found in two of the cryptic AUGs. Two oligopyrimidine-rich tracts upstream to the conserved either cryptic or authentic AUG in the 5'-UTR of pestivirus were identified and 83.3% of their nucleotide sequences are complementary to the consensus sequence at the 3' terminus of eucaryotic 18S rRNA. A secondary structure model for the 5'-UTR of pestivirus was predicted. Nucleotide sequence comparison among five pestiviruses led to the identification of a variable region and a conserved region in the 3'-UTR. A deletion of 41 nucleotides was found within the variable region in Osloss. A secondary structure model for the 3'-UTR was also predicted. The structural similarity of the 5'-UTR between pestiviruses and picornaviruses and hepatitis C viruses was demonstrated and the possible implications of features of the 5' and 3'-UTR of pestiviruses are discussed.
在瘟病毒基因组保守的5'非翻译区（UTR）内，鉴定出三个高度可变区。在多蛋白起始密码子之前，多个隐蔽AUG密码子和几个小开放阅读框是所有五种瘟病毒的特征。对AUG上下文的检查表明，瘟病毒的多蛋白起始AUG在有效翻译起始方面的上下文较弱。在两个隐蔽AUG中发现了最有利的上下文。在瘟病毒5'-UTR中保守的隐蔽或真实AUG上游鉴定出两个富含寡嘧啶的区域，其核苷酸序列的83.3%与真核18S rRNA 3'末端的共有序列互补。预测了瘟病毒5'-UTR的二级结构模型。五种瘟病毒之间的核苷酸序列比较导致在3'-UTR中鉴定出一个可变区和一个保守区。在Osloss的可变区内发现了41个核苷酸的缺失。还预测了3'-UTR的二级结构模型。证明了瘟病毒与小RNA病毒和丙型肝炎病毒在5'-UTR上的结构相似性，并讨论了瘟病毒5'和3'-UTR特征的可能影响。
