Dikalov S, Kirilyuk I, Grigor'ev I
Institute of Chemical Kinetics and Combustion, Institute of Organic Chemistry, Novosibirsk, Russia.
Biochem Biophys Res Commun. 1996 Jan 17;218(2):616-22. doi: 10.1006/bbrc.1996.0109.
Spin trapping with 2H-imidazole-1-oxides (2,2,4-trimethyl-2H-imidazole-1-oxide (TMIO); 4-carboxy-2,2-dimethyl-2H-imidazole-1-oxide, potassium salt (CDMIO); 2,2-dimethyl-4-phenyl-2H-imidazole-1-oxide (DMPIO)) was studied. It was found that these compounds form spin adducts with different short lived free radicals, such as OH, CH3, CH2OH, CH3(CH)OH, C(CH3)3O, HOCH2CH2S, glutathionyl and cysteinyl radicals. 2H-imidazole-1-oxides did not trap superoxide radical. Therefore, the detection of OH and thiol radicals is not complicated by the presence of superoxide radical. It was shown that 2H-imidazole-1-oxides are effective scavengers of peroxynitrite forming the same spin adducts as with OH-radicals. Thus, TMIO, CDMIO and DMPIO can be used for spin trapping studies in chemical and biological systems.
研究了用2H-咪唑-1-氧化物（2,2,4-三甲基-2H-咪唑-1-氧化物（TMIO）；4-羧基-2,2-二甲基-2H-咪唑-1-氧化物钾盐（CDMIO）；2,2-二甲基-4-苯基-2H-咪唑-1-氧化物（DMPIO））进行自旋捕获。发现这些化合物与不同的短寿命自由基形成自旋加合物，如OH、CH3、CH2OH、CH3(CH)OH、C(CH3)3O、HOCH2CH2S、谷胱甘肽基和半胱氨酸基自由基。2H-咪唑-1-氧化物不捕获超氧自由基。因此，超氧自由基的存在不会使OH和硫醇自由基的检测复杂化。结果表明，2H-咪唑-1-氧化物是过氧亚硝酸盐的有效清除剂，形成与OH自由基相同的自旋加合物。因此，TMIO、CDMIO和DMPIO可用于化学和生物系统中的自旋捕获研究。
