Chao C F, Chiou S T, Jeng H, Chang W C
Institute of Biochemical Sciences, National Taiwan University, Taipei, R.O.C.
Biochem Biophys Res Commun. 1996 Jan 17;218(2):623-8. doi: 10.1006/bbrc.1996.0110.
We have isolated a small porcine seminal protein called SMI-1. Our results from peptide sequence, amino acid composition and mass spectral analyses reveal that SMI-1 is identical to porcine beta-microseminoprotein, a protein with unknown function. We also report here that this protein inhibits competitively the activity of Na+,K(+)-ATPase purified from porcine cerebral cortex in a dose-dependent manner. The inhibitory effect could be reversed by the addition of ATP. The half-maximal inhibition was achieved at an inhibitor concentration of 90 microM.
