Blasiak J, Kleinwächter V, Walter Z, Zaludová R
Department of Molecular Genetics, University of Lodz, Poland.
Z Naturforsch C J Biosci. 1995 Nov-Dec;50(11-12):820-3. doi: 10.1515/znc-1995-11-1213.
The interaction of an organophosphorus insecticide methylparathion (O,O-dimethyl O-4-nitrophenyl phosphorothioate) with double-stranded DNA was characterized by UV and circular dichroism (CD) spectroscopy. Two kinds of DNA were employed: calf thymus DNA (CT DNA) and a synthetic two-stranded oligomer of sequence 5'-d(TTGGATCCGAATTCAAGCTT)-3'. Melting curves and CD spectra were taken for the DNAs in the presence of the insecticide at methylparathion/DNA base pair molar ratio of 0.5. The insecticide evoked a decrease of the melting temperature and a broadening of the transition range for CT DNA. Similar effects were observed for the synthetic oligomer but they were less pronounced than in the case of CT DNA. Methylparathion evoked a slight shift and an increase in the amplitude of the negative band in the CD spectra of both DNAs. Obtained results indicate that methylparathion may perturb the thermal stability and conformation of DNA, which is an evidence that the insecticide has an ability to interact directly with DNA.
通过紫外光谱和圆二色光谱（CD）对有机磷杀虫剂甲基对硫磷（O,O-二甲基-O-4-硝基苯基硫代磷酸酯）与双链DNA的相互作用进行了表征。使用了两种DNA：小牛胸腺DNA（CT DNA）和序列为5'-d(TTGGATCCGAATTCAAGCTT)-3'的合成双链寡聚物。在甲基对硫磷/DNA碱基对摩尔比为0.5的情况下，测定了杀虫剂存在时DNA的熔解曲线和CD光谱。该杀虫剂导致CT DNA的熔解温度降低，转变范围变宽。在合成寡聚物中也观察到了类似的效应，但不如CT DNA明显。甲基对硫磷使两种DNA的CD光谱中的负带出现轻微位移并使振幅增加。所得结果表明，甲基对硫磷可能会扰乱DNA的热稳定性和构象，这证明该杀虫剂具有直接与DNA相互作用的能力。
