Zhang L, Goldman J E
Department of Pathology, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA.
Neuron. 1996 Jan;16(1):47-54. doi: 10.1016/s0896-6273(00)80022-7.
The traditional view of the external granular layer of the cerebellar cortex giving rise to interneurons has been challenged by recent studies with quail-chick chimeras. To clarify the time and site of origins of interneurons, a retrovirus carrying the beta-galactosidase gene was injected into the deep cerebellar tissue or external granular layer of postnatal day 4/5 rats to label dividing progenitors. After deep cerebellar tissue injection, unipolar cells were found initially in white matter at 2 days postinjection and subsequently in the internal granule and molecular layers 4-6 days postinjection. Morphologically defined basket, stellate, and Golgi neurons were clearly identified by 20 days postinjection. In contrast, retroviral labeling of cells in the external granular layer produced only granule neurons in the internal granule layer. Thus, dividing progenitors in the cerebellar white matter migrate through the white matter into the cortex before differentiating into a variety of cortical interneurons.
小脑皮质外颗粒层产生中间神经元的传统观点受到了近期鹌鹑 - 鸡嵌合体研究的挑战。为了阐明中间神经元的起源时间和部位，将携带β - 半乳糖苷酶基因的逆转录病毒注射到出生后第4/5天大鼠的小脑深部组织或外颗粒层，以标记正在分裂的祖细胞。在小脑深部组织注射后，注射后2天在白质中最初发现单极细胞，随后在注射后4 - 6天在内颗粒层和分子层中发现。注射后20天可清楚地识别出形态学上定义的篮状、星状和高尔基神经元。相比之下，外颗粒层细胞的逆转录病毒标记仅在内颗粒层产生颗粒神经元。因此，小脑白质中正在分裂的祖细胞在分化为多种皮质中间神经元之前，先通过白质迁移到皮质。
