Fitzpatrick K A, Gorski S M, Ursuliak Z, Price J V
Institute of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC, Canada.
Mech Dev. 1995 Oct;53(2):171-83. doi: 10.1016/0925-4773(95)00432-z.
The spatial and temporal expression of seven Drosophila protein tyrosine phosphatase genes during oogenesis was examined by whole mount in-situ hybridization of antisense RNA probes to ovaries. Our observations indicate diverse expression patterns consistent with multiple roles for protein tyrosine phosphatases in the ovary. DPTP99A and corkscrew transcripts are expressed in follicle cells, consistent with possible roles in the EGF receptor signaling pathway. Transcripts from corkscrew and DPTP10D are detected in the germline during oogenesis and localized to the oocyte during egg chamber development. Localization of the two transcripts is disrupted by mutations in egalitarian and Bicaudal D. DLAR and DPTP4E transcripts are found in the germline during the same developmental stages as DPTP10D transcripts, but their transcripts are not localized to the oocyte. DPTP61F transcription is detected only after stage 6 of oogenesis. After stage 10B these transcripts are transported to the oocyte; thus ovarian transcription of DPTP61F may reflect a maternal contribution of the mRNA for later use during embryogenesis. DPTP69D transcripts are sequestered in the nucleus from stage 7 to stage 10, and then released to the cytoplasm. Our observations suggest that the export of DPTP69D mRNA from the nucleus is temporally regulated during oogenesis.
通过将反义RNA探针与卵巢进行整体原位杂交，研究了7个果蝇蛋白酪氨酸磷酸酶基因在卵子发生过程中的时空表达。我们的观察结果表明，其表达模式多样，这与蛋白酪氨酸磷酸酶在卵巢中的多种作用相一致。DPTP99A和corkscrew转录本在卵泡细胞中表达，这与它们可能在表皮生长因子（EGF）受体信号通路中发挥的作用相一致。在卵子发生过程中，corkscrew和DPTP10D的转录本在生殖系中被检测到，并在卵室发育过程中定位于卵母细胞。这两种转录本的定位会因平等基因（egalitarian）和双尾D（Bicaudal D）的突变而受到破坏。在与DPTP10D转录本相同的发育阶段，在生殖系中发现了DLAR和DPTP4E转录本，但它们的转录本并不定位于卵母细胞。DPTP61F的转录仅在卵子发生的6期之后被检测到。在10B期之后，这些转录本被转运到卵母细胞中；因此，DPTP61F的卵巢转录可能反映了mRNA的母体贡献，以供胚胎发育后期使用。DPTP69D转录本在7期到10期被隔离在细胞核中，然后释放到细胞质中。我们的观察结果表明，在卵子发生过程中，DPTP69D mRNA从细胞核的输出受到时间调控。
