Levin S, Nudelman R, Reschiglian P, Pasti L
Pharmaceutical Chemistry Department, School of Pharmacy, The Hebrew University of Jerusalem, Israel.
J Pharm Biomed Anal. 1995 Jun;13(7):869-77. doi: 10.1016/0731-7085(95)01313-a.
A quasi-empirical approach to the simulation of fractograms was examined to verify that the elution behavior of emulsions in power-based field programmed sedimentation field-flow fractionation (SdFFF) is consistent and predictable. The approach was applied to Intralipid, a commercial soybean emulsion and to an investigational medium chain triglyceride emulsion. The simulations predicted the fractograms that were obtained under various conditions of field strength, field decay and velocity of the suspending fluid, using distribution parameters obtained from one preliminary measurement of size distribution profile. Predicted fractograms were compared to experimental ones, under various fractionating powers. Good agreement was observed in most cases, in which interference of the secondary relaxation effects was not effective. The agreement confirmed the applicability of the approach to emulsions and that the simulations can be used instead of actual experiments for the optimization of their characterization by power-programmed SdFFF.
研究了一种用于模拟分馏图的准经验方法，以验证乳液在基于功率的场编程沉降场流分级法（SdFFF）中的洗脱行为是一致且可预测的。该方法应用于市售大豆乳液英脱利匹特以及一种研究用中链甘油三酯乳液。使用从一次尺寸分布曲线的初步测量中获得的分布参数，模拟预测了在不同场强、场衰减和悬浮液流速条件下获得的分馏图。在不同分级功率下，将预测的分馏图与实验分馏图进行了比较。在大多数情况下观察到了良好的一致性，其中二次弛豫效应的干扰无效。这种一致性证实了该方法对乳液的适用性，并且模拟可用于替代实际实验，以通过功率编程SdFFF优化乳液的表征。
