Henrion D, Bevan J A
Institut National de la Santé et de la Recherche Médicale Unit 141, Hôpital Lariboisière, Paris, France.
J Vasc Res. 1995 Nov-Dec;32(6):413-22. doi: 10.1159/000159117.
The infusion of physiological salt solution into the lumen of ring segments of the isolated rabbit facial vein induces an increase in vessel wall tone. Changes in 22Na+ and 45Ca2+ net uptake as well as 22Na+ unidirectional efflux in response to flow in this vessel were compared to the response to histamine (1 microM) and to angiotensin II (0.1 microM). 22Na+ net uptake per unit force developed in response to flow was 7.9-fold greater than that for histamine (1 microM) and twice that for angiotensin II (0.1 microM). In comparison, 45Ca2+ net uptake per unit force in response to flow, histamine and K+ were similar. Flow-induced contraction and the related 45Ca2+ and 22Na+ net uptake were decreased by amiloride (30 microM) and methyl isobutyl amiloride (30 microM) without changes in 45Ca2+ or 22Na+ net uptake per unit force. Ouabain (10 microM) enhanced flow-induced contraction and the related 22Na+ net uptake. Both ouabain (10 microM) and Bay K 8644 (1 microM) increased flow-induced contraction and the related 45Ca2+ net uptake whereas they decreased the 45Ca2+ net uptake per unit force. Flow, histamine and angiotensin II increased the unidirectional efflux of 22Na+. This increase in 22Na+ unidirectional efflux was attenuated by ouabain (10 microM). These experiments demonstrate that the increases in wall force due to flow, histamine and angiotensin II are associated with increases in 22Na+ net uptake and unidirectional efflux. Flow preferentially promotes the entry of Na+, compared to histamine and angiotensin II.
将生理盐溶液注入离体兔面静脉的环段管腔内会导致血管壁张力增加。将该血管中因血流引起的22Na+和45Ca2+净摄取量以及22Na+单向流出量的变化与组胺（1微摩尔）和血管紧张素II（0.1微摩尔）引起的反应进行了比较。因血流产生的每单位力的22Na+净摄取量比组胺（1微摩尔）引起的高7.9倍，是血管紧张素II（0.1微摩尔）引起的两倍。相比之下，因血流、组胺和钾引起的每单位力的45Ca2+净摄取量相似。氨氯吡咪（30微摩尔）和甲基异丁基氨氯吡咪（30微摩尔）可降低血流诱导的收缩以及相关的45Ca2+和22Na+净摄取量，而每单位力的45Ca2+或22Na+净摄取量无变化。哇巴因（10微摩尔）增强了血流诱导的收缩以及相关的22Na+净摄取量。哇巴因（10微摩尔）和Bay K 8644（1微摩尔）均增加了血流诱导的收缩以及相关的45Ca2+净摄取量，而它们降低了每单位力的45Ca2+净摄取量。血流、组胺和血管紧张素II增加了22Na+的单向流出量。哇巴因（10微摩尔）减弱了这种22Na+单向流出量的增加。这些实验表明，由血流、组胺和血管紧张素II引起的壁力增加与22Na+净摄取量和单向流出量的增加有关。与组胺和血管紧张素II相比，血流优先促进Na+的进入。
