查尔酮合酶基因在大肠杆菌中的分子克隆、测序及表达

Molecular cloning, sequencing, and expression in Escherichia coli of the chalcone synthase gene.

作者信息

Shao L, Li Y, Pan A, Cheng Z, Chen M

机构信息

National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing, China.

出版信息

Chin J Biotechnol. 1995;11(2):131-5.

PMID:8562849

Abstract

Flavonoids form a class of secondary metabolites that are abundant in all higher plants. They serve important functions in flower pigmentation. Chalcone Synthase (CHS) is a key enzyme in the biosynthesis of all classes of flavonoids; therefore, variation of the expression of CHS might change the color of flowers. We cloned CHS-A gene from Petunia hybrida, which has 99% sequence homology with those that have been cloned abroad. The sequence of the coding region is 1170bp and encodes a protein of 39kDa. CHS-A gene is also expressed high-efficiently in E. coli. All this work establishes a sound basis for future research on the impact of the CHS-A gene on flower pigmentation.

摘要

类黄酮是一类在所有高等植物中都大量存在的次生代谢产物。它们在花朵色素沉着中发挥着重要作用。查尔酮合酶（CHS）是所有类黄酮生物合成中的关键酶；因此，CHS表达的变化可能会改变花朵的颜色。我们从矮牵牛中克隆了CHS - A基因，其与国外已克隆的基因具有99%的序列同源性。编码区序列为1170bp，编码一个39kDa的蛋白质。CHS - A基因在大肠杆菌中也能高效表达。所有这些工作为今后研究CHS - A基因对花朵色素沉着的影响奠定了坚实的基础。