Zhong J, Yoshida M, Yoshida T
Research Institute of Biochemical Engineering, East China University of Science and Technology, Shanghai, China.
Chin J Biotechnol. 1995;11(2):143-7.
Effects of biological factors (i.e., size of cell aggregates, subculture period, and inoculum density) on cell growth and production of anthocyanin pigment (a secondary metabolite) by suspended cultures of Perilla frutescens plant cells were investigated on a flask scale. It was found that anthocyanin content was lower in a culture inoculated with cells of size greater than 250 microns, compared with unscreen-sized cells or cells smaller than 250 microns. Subculture periods of 7 and 10 days were suitable for the cell cultures since favorable growth and a high level of anthocyanin content were maintained during a long period of subculture. An inoculum density (wet cells) of 50 g/L was the best for both the cell growth and pigment accumulation in flask culture of P. frutescens cells.
在摇瓶规模下，研究了生物因素（即细胞聚集体大小、传代培养时间和接种密度）对紫苏植物细胞悬浮培养中细胞生长及花青素色素（一种次生代谢产物）产量的影响。结果发现，与未筛选大小的细胞或小于250微米的细胞相比，接种大于250微米细胞的培养物中花青素含量较低。7天和10天的传代培养时间适用于细胞培养，因为在长时间传代培养过程中能保持良好的生长和高水平的花青素含量。50 g/L的接种密度（湿细胞）对紫苏细胞摇瓶培养中的细胞生长和色素积累均最为有利。
