Krizanac-Bengez L, Boranic M
Rudjer Boskovic Institute, Zagreb, Croatia.
Biomed Pharmacother. 1995;49(7-8):375-80. doi: 10.1016/0753-3322(96)82668-0.
Enkephalinase-blocking agent thiorphan was added to long-term cultures of mouse bone marrow cells at the time of culture initiation (time 0) or 2 weeks thereafter, when the stromal layer appears. Cellularity, cell morphology (in cytospin smears) and the yield of granulocyte-macrophage progenitor cells (GM-CFC assay in agar) were recorded. Low concentrations of thiorphan accelerated recovery of the cultures after an initial drop of the cell count. Expansion and maturation of the granulocytic lineage was promoted, with parallel decline of the GM-CFC yield. Thiorphan probably interfered with the activity of enkephalinase (endopeptidase 24.11) in the cultures. That enzyme is the CD10 surface marker (CALLA) of lymphoid, myeloid and stromal elements.
在培养开始时（时间0）或两周后（此时出现基质层），将脑啡肽酶阻断剂硫磷酰胺添加到小鼠骨髓细胞的长期培养物中。记录细胞密度、细胞形态（在细胞涂片上）和粒细胞-巨噬细胞祖细胞的产量（琼脂中的GM-CFC测定）。低浓度的硫磷酰胺在细胞计数最初下降后加速了培养物的恢复。促进了粒细胞系的扩增和成熟，同时GM-CFC产量平行下降。硫磷酰胺可能干扰了培养物中脑啡肽酶（内肽酶24.11）的活性。该酶是淋巴、髓样和基质成分的CD10表面标志物（CALLA）。
