Therapeutic effect of CDDP and VP-16 against human bladder cancers.

In order to evaluate the anticancer effect of combination chemotherapy (CDDP and etoposide) against human bladder cancer xenografts transplanted in nude mice, we tried an experimental chemotherapy. The tumor was aseptically cut and subcutaneously transplanted into the backs of the nude mice. When the weight of each tumor reached 300 mg, CDDP (day 1; 4 mg/kg) and etoposide (day 1-5; 2 mg/kg) were given intraperitoneally as single agent or in combination and repeated every 3 weeks. The estimated weight (mg) was obtained by (larger diam. x smaller diam.2) x 1/2. A continuous significant effect was observed in CDDP combined with etoposide against bladder tumor. Therefore, these results suggest that the combination chemotherapy using CDDP with etoposide is an efficacious treatment against human bladder cancer.