Suppr超能文献

D-半乳糖胺对大鼠肝细胞中糖核苷酸和糖缀合物合成的影响。

Influence of D-galactosamine on the synthesis of sugar nucleotides and glycoconjugates in rat hepatocytes.

作者信息

Pels Rijcken W R, Ferwerda W, Van den Eijnden D H, Overdijk B

机构信息

Department of Medical Chemistry, Vrije Universiteit, Amsterdam, The Netherlands.

出版信息

Glycobiology. 1995 Jul;5(5):495-502. doi: 10.1093/glycob/5.5.495.

Abstract

Rat hepatocytes were incubated in the presence of a high concentration of the hepatopathogenic agent D-galactosamine (GalN), and the effect on the cellular concentrations of pyrimidine nucleotides and nucleotide sugars was determined. The UTP pool became depleted. The pools of UMP and CMP in RNA decreased to 72%, indicative for an inhibition of RNA synthesis. UDP-HexNAc (where HexNAc is GlcNAc+GalNAc) and UDP-HexN (where HexN is GlcN+GalN) levels increased, and those of UDP-hexose and UDP-GlcA (where GlcA is glucuronic acid) decreased. The cellular concentration of CTP did not change, whereas that of CMP-NeuAc (where NeuAc is N-acetylneuraminic acid) showed a 2-fold increase. Labelling with [14C]orotic acid and [3H]cytidine showed that the metabolic flow via the de novo pathway was not changed. The depletion of the so-called overflow pool of UTP [Pels Rijcken et al., Biochem. J., 293, 207-213, 1993] caused a release of the feedback inhibition by UTP and thus an increased flow through the salvage pathway. Finally, it appeared that GalN, when added to hepatocytes, gives rise to a pool of UDP-GlcNAc (where GlcNAc is N-acetylglucosamine) that is separate from the pool of UDP-GlcNAc that is derived from GlcN.

摘要

将大鼠肝细胞置于高浓度的致肝病变剂D-半乳糖胺(GalN)存在的环境中培养,测定其对嘧啶核苷酸和核苷酸糖细胞浓度的影响。UTP库耗竭。RNA中的UMP和CMP库降至72%,表明RNA合成受到抑制。UDP-HexNAc(其中HexNAc为GlcNAc+GalNAc)和UDP-HexN(其中HexN为GlcN+GalN)水平升高,而UDP-己糖和UDP-GlcA(其中GlcA为葡萄糖醛酸)水平降低。CTP的细胞浓度未发生变化,而CMP-NeuAc(其中NeuAc为N-乙酰神经氨酸)的细胞浓度增加了2倍。用[14C]乳清酸和[3H]胞苷标记表明,从头合成途径的代谢通量未发生变化。UTP所谓溢流库的耗竭[Pels Rijcken等人,《生物化学杂志》,293,207-213,1993]导致UTP反馈抑制的解除,从而使补救途径的通量增加。最后发现,当将GalN添加到肝细胞中时,会产生一个与源自GlcN的UDP-GlcNAc库(其中GlcNAc为N-乙酰葡糖胺)分开的UDP-GlcNAc库。

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验