Roberts D L, Runquist J A, Miziorko H M, Kim J J
Department of Biochemistry, Medical College of Wisconsin, Milwaukee 53226, USA.
Protein Sci. 1995 Nov;4(11):2442-3. doi: 10.1002/pro.5560041126.
A recombinant form of Rhodobacter sphaeroides phosphoribulokinase (PRK), expressed in Escherichia coli and isolated by affinity chromatography, was crystallized by the sitting drop vapor diffusion technique using NH4H2PO4 (pH 5.6) as the precipitating agent. PRK crystallizes in the cubic space group P432, with unit cell parameters a = b = c = 129.55 A. Based on the assumption of one 32-kDa monomer per asymmetric unit, the Vm value is 2.83 A3/Da. The octameric molecular symmetry is consistent with two planar tetramers stacked in a nearly eclipsed arrangement. A native data set has been collected to 2.6 A resolution.
一种重组形式的球形红细菌磷酸核酮糖激酶（PRK），在大肠杆菌中表达并通过亲和层析分离，使用NH4H2PO4（pH 5.6）作为沉淀剂，通过坐滴气相扩散技术结晶。PRK在立方空间群P432中结晶，晶胞参数a = b = c = 129.55 Å。基于每个不对称单元有一个32 kDa单体的假设，Vm值为2.83 Å3/Da。八聚体分子对称性与两个平面四聚体以几乎重叠的排列方式堆叠一致。已收集到分辨率为2.6 Å的天然数据集。
