Hajós F, Zilles K
Department of Anatomy and Histology, University of Veterinary Science, Budapest, Hungary.
Neurobiology (Bp). 1995;3(1):3-11.
Coronal vibratome sections of the rat brain were immunostained for glial fibrillary acidic protein (GFAP) a major cytoskeletal protein typical for astrocytes. Using computer-assisted image analysis of whole sections, a substantial heterogeneity in regional staining intensities was pointed out. The middle layers of the neocortex, the thalamus and caudate-putamen and the tectal and tegmental part of the mesencephalon were found to show no detectable staining, while peak staining intensities were measured for the pallidum, septal triangular nucleus, hippocampus, medial geniculate nucleus and interpeduncular nucleus. In some of the negative areas neural lesioning induced the appearance of GFAP immunoreaction parallel to an up-regulation of GFAP synthesis. On this basis it was assumed that GFAP immunoreactivity is dormant in these astrocytes and plastic changes in the neuropil trigger its expression.
对大鼠脑的冠状振动切片进行胶质纤维酸性蛋白（GFAP）免疫染色，GFAP是星形胶质细胞典型的主要细胞骨架蛋白。通过对整个切片进行计算机辅助图像分析，发现区域染色强度存在显著异质性。新皮层中层、丘脑、尾状核-壳核以及中脑顶盖和被盖部分未检测到染色，而苍白球、隔三角核、海马、内侧膝状体核和脚间核的染色强度达到峰值。在一些阴性区域，神经损伤诱导了GFAP免疫反应的出现，同时伴随着GFAP合成的上调。在此基础上推测，这些星形胶质细胞中的GFAP免疫反应性处于休眠状态，神经毡的可塑性变化触发了其表达。
