Kálmán M, Tuba A
1st Department of Anatomy, Semmelweis University of Medicine, Budapest, Hungary.
Neurobiology (Bp). 1995;3(2):165-74.
The transplantation of embryonic dopaminergic tissue is an approach to the therapy of parkinsonism. In this study we compared the development of the rat embryonic ventral mesencephalon transplanted together or without striatal tissue into the cerebral cortex of adult rats. After one month, the survival ratio was 17 of 44 when striatal tissue was co-grafted, while only 4 of 45 transplants survived when the co-graft was omitted. In the mesencephalic graft, the appearance of tyrosine hydroxylase-contaning cells was investigated by immunohistochemical method. Applying a fluorescent tracer dye, we demonstrated the growth of neural fibers from the mesencephalic tissue to the striatal co-graft. In situ, the embryonic striatal tissue is the natural target area for the developing nigrostriatal pathway. The possible role of trophic factors and postsynaptic target surfaces provided by the co-graft is discussed as basis of the adjuvant effect.
胚胎多巴胺能组织移植是治疗帕金森病的一种方法。在本研究中，我们比较了大鼠胚胎腹侧中脑与纹状体组织一起或不与纹状体组织一起移植到成年大鼠大脑皮层后的发育情况。一个月后，联合移植纹状体组织时，44个移植体中有17个存活，而未进行联合移植时，45个移植体中只有4个存活。在中脑移植体中，通过免疫组织化学方法研究了含酪氨酸羟化酶细胞的出现情况。应用荧光示踪染料，我们证明了神经纤维从中脑组织生长到纹状体联合移植体。在原位，胚胎纹状体组织是发育中的黑质纹状体通路的天然靶区。讨论了联合移植提供的营养因子和突触后靶表面的可能作用，作为辅助作用的基础。
