D'Cruz O J, Haas G G
Department of Obstetrics and Gynecology, University of Oklahoma Health Sciences Center, Oklahoma City 73190, USA.
Res Commun Mol Pathol Pharmacol. 1995 Jun;88(3):243-70.
We tested the hypothesis that binding of rabbit isoimmune antisperm Fab fragments to rabbit sperm prior to artificial insemination could protect the sperm from isoimmune attack in vivo and lead to normal pregnancy and healthy offspring in isoimmune does. Twenty-four female rabbits and 4 males were used. Twelve does were immunized with rabbit sperm to induce isoimmunity and 12 does immunized with adjuvant/saline served as controls. Following 5 immunizations, the immune serum IgG fraction and its Fab fragments were prepared. The does from the control group and isoimmune group were artificially inseminated following the 6th and 7th immunization with untreated sperm or sperm pretreated with antisperm IgG or Fab in quadruplicates and allowed to complete a pregnancy. Three fertility trials were performed to investigate the therapeutic efficacy of Fab-coated sperm to restore fertility in isoimmune does. In the 3 fertility trials, none of the isoimmune does inseminated with untreated sperm had a successful pregnancy. By contrast, in 3 cases, rabbit sperm pretreated with antisperm Fab were able to fertilize in vivo with successful pregnancy in a control and isoimmune doe resulting in normal offspring. These results demonstrate that in vitro treatment of sperm with antisperm Fab fragments had a protective effect from isoimmune attack in vivo. The successful use of Fab fragments for reversal of antisperm antibody-mediated infertility observed in the isoimmune rabbit model offers the prospect of a new means of restoring fertility in some isoimmune women.
在人工授精前，兔同种免疫抗精子Fab片段与兔精子结合能够在体内保护精子免受同种免疫攻击，并使同种免疫的母兔正常怀孕并产下健康后代。实验使用了24只雌兔和4只雄兔。12只母兔用兔精子进行免疫以诱导同种免疫，12只用佐剂/盐水免疫的母兔作为对照。经过5次免疫后，制备免疫血清IgG组分及其Fab片段。在第6次和第7次免疫后，对照组和同种免疫组的母兔分别用未处理的精子、用抗精子IgG或Fab预处理的精子进行人工授精，每组重复4次，然后使其完成妊娠。进行了三项生育试验，以研究Fab包被的精子恢复同种免疫母兔生育能力的治疗效果。在这三项生育试验中，用未处理精子进行人工授精的同种免疫母兔均未成功怀孕。相比之下，在3个案例中，用抗精子Fab预处理的兔精子能够在体内受精，一只对照母兔和一只同种免疫母兔成功怀孕并产下正常后代。这些结果表明，体外使用抗精子Fab片段处理精子对其在体内免受同种免疫攻击具有保护作用。在同种免疫兔模型中观察到Fab片段成功用于逆转抗精子抗体介导的不孕症，这为恢复一些同种免疫女性的生育能力提供了一种新方法。
