Okayama Y, Brzezińska-Błaszczyk E, Kuna P, Kaplan A P, Church M K
Immunopharmacology Group, Southampton General Hospital, UK.
Clin Exp Allergy. 1995 Sep;25(9):890-5. doi: 10.1111/j.1365-2222.1995.tb00032.x.
A field of study which has attracted much recent interest is the ability of mononuclear cells and neutrophils to interact with histamine releasing cells by production of specific histamine releasing factors (HRFs). However, almost all of these studies have been performed on basophils rather than human mast cells.
We have investigated the effects of lyophilized fractions of HRF preparations on histamine release from human skin and lung mast cells.
Lyophilized fractions of HRF preparations include crude supernatant from mononuclear cell/platelet (crude), void peak from anion exchange chromatography column (void), second peak from anion exchange chromatography (peak 2), neutrophil-activating peptide-2 (NAP-2), which was purified from void peak at molecular weight of 8-12 kDa, and monocyte chemotactic-activating factor (MCAF). Mast cells were enzymatically dispersed.
Crude (24.2 micrograms/mL-2.42 mg/mL), void (5.4 micrograms/mL-0.54 mg/mL), peak 2 (3.5 micrograms/mL-0.35 mg/mL), and NAP-2 (1-20 micrograms/mL) failed to release histamine from lung mast cells. In skin mast cells, only higher concentrations of crude and void caused minimal release of histamine. MCAF up to micromolar concentrations failed to have an effect on mast cells from either source. However, these HRFs induced histamine release from human basophils. We also explored whether HRFs and stem cell factor could act as either priming agents for each other or for anti-IgE. The response of skin mast cells to all these preparations was not enhanced by preincubation in stem cell factor at 1 ng/mL, nor did the HRFs and MCAF enhance the response of skin mast cells to anti-IgE.
These results suggest that these HRFs have no significant effect on dispersed human cutaneous and lung mast cells.
单核细胞和中性粒细胞通过产生特异性组胺释放因子（HRF）与组胺释放细胞相互作用的能力是近年来备受关注的一个研究领域。然而，几乎所有这些研究都是在嗜碱性粒细胞而非人肥大细胞上进行的。
我们研究了HRF制剂冻干组分对人皮肤和肺肥大细胞组胺释放的影响。
HRF制剂冻干组分包括单核细胞/血小板粗提上清液（粗提物）、阴离子交换色谱柱的空体积峰（空体积峰）、阴离子交换色谱的第二个峰（峰2）、从中性粒细胞激活肽-2（NAP-2），其从分子量为8-12 kDa的空体积峰中纯化得到，以及单核细胞趋化激活因子（MCAF）。肥大细胞经酶分散处理。
粗提物（24.2微克/毫升至2.42毫克/毫升）、空体积峰（5.4微克/毫升至0.54毫克/毫升）、峰2（3.5微克/毫升至0.35毫克/毫升）和NAP-2（1至20微克/毫升）均未能从肺肥大细胞释放组胺。在皮肤肥大细胞中，只有更高浓度的粗提物和空体积峰引起了极少量的组胺释放。高达微摩尔浓度的MCAF对两种来源的肥大细胞均无作用。然而，这些HRF可诱导人嗜碱性粒细胞释放组胺。我们还探讨了HRF和干细胞因子是否可相互作为引发剂或作为抗IgE的引发剂。皮肤肥大细胞对所有这些制剂的反应在1纳克/毫升的干细胞因子中预孵育后并未增强，HRF和MCAF也未增强皮肤肥大细胞对抗IgE的反应。
这些结果表明，这些HRF对分散的人皮肤和肺肥大细胞无显著影响。
