用于放射免疫分析的高质量碘-125标记垂体人促卵泡激素（hFSH）的制备：酶促碘化与氯胺-T碘化的比较
Preparation of high-quality iodine-125-labelled pituitary human follicle-stimulating hormone (hFSH) for radioimmunoassay: comparison of enzymatic and chloramine-T iodination.
作者信息
Pinto H, Lerario A C, Torres de Toledo e Souza I, Wajchenberg B L, Mattar E, Pieroni R R
出版信息
Clin Chim Acta. 1977 Apr 1;76(1):25-34. doi: 10.1016/0009-8981(77)90115-2.
PMID:856502
Abstract
A method is described for the enzymatic radioiodination of human follicle-stimulating hormone (hFSH) by a system consisting of lactoperoxidase, hydrogen peroxide and Na125I. It was compared with the Chloramine-T modified technique. A satisfactory specific activity of the labelled hormone was obtained with the enzymatic iodination with much greater immunoreactivity was stability than after Chloramine-T.
摘要
描述了一种通过由乳过氧化物酶、过氧化氢和Na125I组成的系统对人促卵泡激素（hFSH）进行酶促放射性碘化的方法。将其与氯胺-T改良技术进行了比较。酶促碘化获得了令人满意的标记激素比活性，其免疫反应性和稳定性比氯胺-T处理后更高。
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