涡虫神经系统中的小清蛋白免疫反应性蛋白
Parvalbumin-immunoreactive proteins in the nervous system of planarians.
作者信息
Hutticher A, Kerschbaum H H, Kainz V, Bito M, Hermann A
机构信息
Department of Animal-Physiology, University of Salzburg, Austria.
出版信息
Cell Mol Neurobiol. 1995 Aug;15(4):401-10. doi: 10.1007/BF02071876.
Abstract
- Using immunological methods, we have identified parvalbumin-like material in the triclade flatworms, Polycelis nigra, Polycelis auriculata, Crenobia alpina, Dugesia tahitiensis, and Dugesia polychroa. 2. Western immunoblot analysis of these five species revealed heat stable parvalbumin-immunoreactive proteins between 32 kD and 44 kD. 3. Proteins at 19 and 32 kD revealed intense labeling with 45Ca2+. 4. Double immunodiffusion of planarian supernatants showed complete fusion of precipitates, indicating immunological relatedness of the parvalbumin-like material among the species investigated. 5. Immunocytochemical studies exhibit parvalbumin-immunoreactive material exclusively in neurons supporting the notion of an early evolutionary appearance of these proteins in the nervous system.
摘要
- 我们利用免疫学方法，在三枝扁形虫（Polycelis nigra、Polycelis auriculata、Crenobia alpina、Dugesia tahitiensis和Dugesia polychroa）中鉴定出了类小白蛋白物质。2. 对这五个物种的蛋白质免疫印迹分析显示，在32 kD至44 kD之间存在热稳定的小白蛋白免疫反应性蛋白。3. 19 kD和32 kD的蛋白质显示出与45Ca2+的强烈结合。4. 涡虫上清液的双向免疫扩散显示沉淀物完全融合，表明在所研究的物种中类小白蛋白物质具有免疫相关性。5. 免疫细胞化学研究表明，小白蛋白免疫反应性物质仅存在于神经元中，支持了这些蛋白质在神经系统中早期进化出现的观点。
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