Dai A, Zhang Z, Niu R
Department of Respiratory Disease, Tongji Hospital, Tonji Medical University, Wuhan.
Zhonghua Jie He He Hu Xi Za Zhi. 1995 Jun;18(3):164-6, 191.
The aim of the study is to investigate whether hypoxia might affect nitric oxide synthase (NOS) activity or mRNA expression of cultured pulmonary artery endothelial cells in pigs by means of NADPH-diaphorase cytochemical stain and DNA-RNA dot blot hybridization respectively. The NOS activity and mRNA expression were highly present in normoxic group as well as 6 and 24 hours serum-free control groups, but significantly lowered in 6 and 24 hours hypoxic groups. The mRNA expression of NOS gene was almost absent in 48 hours hypoxic cells. The results indicate that hypoxia can attenuate the activity and mRNA expression of NOS in cultured pulmonary artery endothelial cells, which may lay an important role in modulating acute hypoxic pulmonary vasocontriction and chronic hypoxic pulmonary arterial hypertension.
本研究旨在分别通过NADPH-黄递酶细胞化学染色和DNA-RNA斑点杂交技术，探讨低氧是否会影响猪肺动脉内皮细胞一氧化氮合酶（NOS）的活性或mRNA表达。正常氧组以及无血清培养6小时和24小时的对照组中，NOS活性和mRNA表达均较高，但在低氧6小时和24小时组中显著降低。在低氧48小时的细胞中，NOS基因的mRNA表达几乎消失。结果表明，低氧可减弱培养的肺动脉内皮细胞中NOS的活性和mRNA表达，这可能在调节急性低氧性肺血管收缩和慢性低氧性肺动脉高压中起重要作用。
