Papadea C, Cate J C
Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston 29425, USA.
Clin Chem. 1996 Jan;42(1):57-63.
The Bio-Rad Variant Hemoglobin Testing System is an automated HPLC analyzer marketed with a Beta-thalassemia Short Program to quantify hemoglobins (Hbs) F and A2 and assist in detecting Hbs A, S, C, D, and E. We evaluated this system to replace several traditional methods for Hb in our hospital laboratory. Analytical performance relevant to quantifying Hbs A, S, C, and F was assessed with blood samples obtained from our local patient population. Studies of precision (CVs < 3%) and analytical limits (% of total Hb) of Hbs A (2-86%), F (1-89%), S (5-90%), and C (3-92%) demonstrated results comparable with or exceeding those of traditional methods. Results for patients' samples (n) for Hbs A (107), F (157), S (128), and C (27) correlated well (r > 0.93) with results by traditional methods. The satisfactory performance and efficiency led us to implement this system for routine quantification of clinically significant Hbs.
伯乐Variant血红蛋白检测系统是一款自动化高效液相色谱分析仪，其销售时配备了β地中海贫血短程序，用于定量血红蛋白（Hb）F和A2，并辅助检测Hb A、S、C、D和E。我们对该系统进行了评估，以取代我院实验室中几种传统的血红蛋白检测方法。我们使用从当地患者群体采集的血样，评估了与定量Hb A、S、C和F相关的分析性能。对Hb A（2-86%）、F（1-89%）、S（5-90%）和C（3-92%）的精密度（CVs<3%）和分析限度（占总Hb的百分比）研究表明，其结果与传统方法相当或优于传统方法。患者样本（n）中Hb A（107）、F（157）、S（128）和C（27）的结果与传统方法的结果相关性良好（r>0.93）。该系统令人满意的性能和效率促使我们将其用于临床上重要血红蛋白的常规定量检测。
