Meese E, Göttert E, Zang K D, Sauter M, Schommer S, Mueller-Lantzsch N
Institut für Humangenetik, Universitätskliniken des Saarlandes, Homburg/Saar, Germany.
Cytogenet Cell Genet. 1996;72(1):40-2. doi: 10.1159/000134157.
The human endogenous retrovirus K10 (HERV-K10) was mapped to human chromosomes using HERV-K10 specific PCR primers on a somatic hybrid mapping panel. A non-random chromosomal location was demonstrated with PCR signals on chromosomes 1, 3, 4, 5, 6, 7, 10, 11, 12, 14, 15, 19, 20, 21, 22 and Y. There was a lack of PCR products on the other chromosomes, even after hybridization with a HERV-K10 specific probe. To further localize the HERV-K10 sequence we used fluorescence in situ hybridization. Chromosomes 1, 3, 6, 7, 10, 11, 12 and 22 were found to contain several HERV-K10 sequences in different regions. The presence of several integration sites on some chromosomes is consistent with previous studies demonstrating 30-50 copies of the HERV-K10 sequence per haploid genome. The mapping information reported in this study will assist the analysis of the biological significance of the HERV-K10 sequence.
利用人内源性逆转录病毒K10（HERV-K10）特异性PCR引物，在体细胞杂种定位板上对HERV-K10进行人染色体定位。在1、3、4、5、6、7、10、11、12、14、15、19、20、21、22号染色体和Y染色体上通过PCR信号证明了其非随机染色体定位。即使与HERV-K10特异性探针杂交后，在其他染色体上仍未检测到PCR产物。为了进一步定位HERV-K10序列，我们采用了荧光原位杂交技术。发现1、3、6、7、10、11、12和22号染色体在不同区域含有多个HERV-K10序列。一些染色体上存在多个整合位点，这与先前研究表明单倍体基因组中存在30 - 50个HERV-K10序列拷贝的结果一致。本研究报告的定位信息将有助于分析HERV-K10序列的生物学意义。
