运动发酵单胞菌胞外蔗糖酶（SacC）在大肠杆菌中的过表达
Overexpression of extracellular sucrase (SacC) of Zymomonas mobilis in Escherichia coli.
作者信息
Kannan R, Pitchaimani K, Gunasekaran P, Aït-Abdelkader N, Baratti J
机构信息
Department of Microbial Technology, School of Biological Sciences, Madurai Kamaraj University, India.
出版信息
FEMS Microbiol Lett. 1995 Nov 1;133(1-2):29-33. doi: 10.1016/0378-1097(95)00327-2.
The extracellular sucrase (SacC) gene of Zymomonas mobilis was overexpressed in Escherichia coli BL21 using the T7 polymerase expression system. A low cell density induction method was designed to have maximum expression, and the conditions (IPTG concentration, ampicillin addition) were optimised to overexpress to the level of more than 60% of the total cellular protein representing SacC protein.
利用T7聚合酶表达系统在大肠杆菌BL21中过表达运动发酵单胞菌的细胞外蔗糖酶（SacC）基因。设计了一种低细胞密度诱导方法以实现最大表达，并对条件（异丙基-β-D-硫代半乳糖苷（IPTG）浓度、氨苄青霉素添加量）进行了优化，以使SacC蛋白的表达量超过总细胞蛋白的60%。
Zotero 插件