Turner S L, Young J P
Department of Biology, University of York, UK.
FEMS Microbiol Lett. 1995 Nov 1;133(1-2):53-8. doi: 10.1111/j.1574-6968.1995.tb07860.x.
The replicator region of the cryptic plasmid pRL8JI from Rhizobium leguminosarum strain 3841 was cloned and sequenced. The recombinant plasmid (pYK3) was selected by function from a partial EcoRI library of total DNA cloned in pSUP202 and shows incompatibility with plasmid pRL8JI when conjugated into R. leguminosarum strains 3841 and its derivative 1062. The cloned insert (approximately 10.5 kb) comprises five EcoRI fragments none of which confers replicative stability when cloned individually. A single 5.0-kb BamHI fragment, that spans all five EcoRI fragments and confers replicative stability on pSUP202 in R. leguminosarum, has been sequenced. This replicator region shows organisational and sequence similarity to the replicator regions of the Agrobacterium plasmids pTiB6S3 and pRiA4b. It has three open reading frames (repA, repB, repC) and a conserved intergenic sequence.
对来自豌豆根瘤菌3841菌株的隐蔽质粒pRL8JI的复制区进行了克隆和测序。重组质粒（pYK3）是从克隆于pSUP202的总DNA的部分EcoRI文库中通过功能筛选得到的，当将其接合到豌豆根瘤菌3841菌株及其衍生物1062中时，显示出与质粒pRL8JI不相容。克隆的插入片段（约10.5 kb）由五个EcoRI片段组成，单独克隆时没有一个片段能赋予复制稳定性。一个5.0 kb的BamHI片段，跨越所有五个EcoRI片段，并赋予pSUP202在豌豆根瘤菌中的复制稳定性，已进行了测序。该复制区与根癌土壤杆菌质粒pTiB6S3和pRiA4b的复制区在组织和序列上具有相似性。它有三个开放阅读框（repA、repB、repC）和一个保守的基因间序列。
