Giardina P C, Foster L A, Toth S I, Roe B A, Dyer D W
Department of Microbiology and Immunology, State University of New York at Buffalo 14214, USA.
Gene. 1995 Dec 29;167(1-2):133-6. doi: 10.1016/0378-1119(95)00659-1.
The alcA gene, essential for the production of the dihydroxamate siderophore, alcaligin, by Bordetella bronchiseptica, was cloned and sequenced. The alcA gene was identified on a 4.7-kb EcoRI genomic fragment adjacent to a Tn5lac transposon insertion that inactivated alcaligin production in strain MBORD846. Analysis of the alcA nucleotide sequence revealed a putative Fur-binding site, suggesting that expression of this gene is repressed by iron. The deduced amino-acid sequence of this open reading frame had significant homology with the Escherichia coli iucD gene product, an enzyme required for biosynthesis of the dihydroxamate siderophore aerobactin.
支气管败血波氏杆菌产生二羟肟酸铁载体（alkaligin）所必需的alcA基因被克隆并测序。在一个与Tn5lac转座子插入相邻的4.7 kb EcoRI基因组片段上鉴定出alcA基因，该转座子插入使MBORD846菌株中的alkaligin产生失活。对alcA核苷酸序列的分析揭示了一个假定的Fur结合位点，表明该基因的表达受铁抑制。这个开放阅读框推导的氨基酸序列与大肠杆菌iucD基因产物具有显著同源性，iucD基因产物是二羟肟酸铁载体气杆菌素生物合成所需的一种酶。
