Labbé J C, Jannatipour M, Rokeach L A
Département de biochimie, Université de Montréal, Québec, Canada.
Gene. 1995 Dec 29;167(1-2):227-31. doi: 10.1016/0378-1119(95)00695-8.
As a first step toward establishing a genetic system for the elucidation of the cellular role(s) of the Ro ribonucleoproteins (RoRNP), we have cloned the gene encoding the homologue of the human 60-kDa Ro protein (Ro60) in Caenorhabditis elegans (Ce). This Ce gene is present as a single copy and contains a 643-codon open reading frame interrupted by three introns. The encoded protein, Rop1p, shares 40% identity and 63% overall similarity with both the human and amphibian Ro60. Recombinant protein has been produced in Escherichia coli and used to elicit anti-Rop1p antibodies. Immunological analysis indicated that the Ro60 epitopes have been poorly conserved. Gene-fusion expression studies in transgenic nematodes will provide a new avenue of research to shed light on the function of these particles.
作为建立用于阐明Ro核糖核蛋白（RoRNP）细胞作用的遗传系统的第一步，我们已经在秀丽隐杆线虫（Ce）中克隆了编码人类60 kDa Ro蛋白（Ro60）同源物的基因。这个Ce基因以单拷贝形式存在，包含一个由三个内含子中断的643密码子开放阅读框。编码的蛋白质Rop1p与人类和两栖动物的Ro60具有40%的同一性和63%的总体相似性。重组蛋白已在大肠杆菌中产生，并用于产生抗Rop1p抗体。免疫分析表明Ro60表位的保守性较差。在转基因线虫中的基因融合表达研究将为揭示这些颗粒的功能提供一条新的研究途径。
