恶臭假单胞菌主要σ因子编码基因rpoD的分析。

Analysis of the rpoD gene encoding the principal sigma factor of Pseudomonas putida.

作者信息

Fujita M, Hanaura Y, Amemura A

机构信息

Radiosotope Center, National Institute of Genetics, Shizuoka, Japan.

出版信息

Gene. 1995 Dec 29;167(1-2):93-8. doi: 10.1016/0378-1119(95)00675-3.

DOI:10.1016/0378-1119(95)00675-3

PMID:8566819

Abstract

The gene (rpoD) encoding the principal sigma factor of Pseudomonas putida (Pp) was cloned and sequenced. The amino-acid sequence deduced from the nucleotide sequence of rpoD contained sequences with significant similarity to the conserved region of the principal sigma factors. In vivo transcriptional analyses revealed that the Pp rpoD is transcribed as a monocistronic mRNA of 2.1 kb and that the transcription of rpoD is under control of the heat-shock (HS) response. The transcription start point (tsp) of the gene was determined and found to be different depending on either normal growth (at 30 degrees C) or HS (at 42 degrees C) conditions. The possible promoter sequences for the principal (sigma 70) and the HS RNA polymerase of Pseudomonas were located in the upstream region of the tsp.

摘要

对恶臭假单胞菌（Pp）主要σ因子的编码基因（rpoD）进行了克隆和测序。从rpoD核苷酸序列推导的氨基酸序列包含与主要σ因子保守区域具有显著相似性的序列。体内转录分析表明，Pp rpoD转录为2.1 kb的单顺反子mRNA，且rpoD的转录受热休克（HS）反应调控。确定了该基因的转录起始点（tsp），发现其在正常生长（30℃）或HS（42℃）条件下有所不同。恶臭假单胞菌主要（σ70）和HS RNA聚合酶的可能启动子序列位于tsp的上游区域。