鉴定五个编码与线粒体NADH-泛醌氧化还原酶的ND亚基等同物的荚膜红细菌基因。

Identification of five Rhodobacter capsulatus genes encoding the equivalent of ND subunits of the mitochondrial NADH-ubiquinone oxidoreductase.

作者信息

Dupuis A, Peinnequin A, Chevallet M, Lunardi J, Darrouzet E, Pierrard B, Procaccio V, Issartel J P

机构信息

Laboratoire de Biochimie, URA CNRS 1130, DBMS, CEA, Grenoble, France.

出版信息

Gene. 1995 Dec 29;167(1-2):99-104. doi: 10.1016/0378-1119(95)00693-1.

DOI:10.1016/0378-1119(95)00693-1

PMID:8566820

Abstract

We previously reported the sequencing of two genes (ndhA and ndhI) encoding two of the subunits of the type-I NADH-ubiquinone oxidoreductase from Rhodobacter capsulatus (Rc). The present paper deals with the cloning and characterization of a chromosomal fragment clustering five new Rc genes which encode subunits of this enzyme. This gene cluster is located immediately downstream from ndhA and ndhI, and also contains two unidentified open reading frames (urf2, urf3). The five genes, nuoJ, nuoK, nuoL, nuoM and nuoN, encode proteins related, respectively, to mitochondrial (mt) subunits ND6, ND4L, ND5, ND4 and ND2. The overall organization of the nuo genes identified in Rc shows similarity to that of the Paracoccus denitrificans (Pd) nqo gene cluster.

摘要

我们之前报道了来自荚膜红细菌（Rc）的编码I型NADH-泛醌氧化还原酶两个亚基的两个基因（ndhA和ndhI）的测序。本文涉及一个染色体片段的克隆和特性分析，该片段聚集了五个新的Rc基因，这些基因编码该酶的亚基。这个基因簇位于ndhA和ndhI的紧邻下游，还包含两个未鉴定的开放阅读框（urf2、urf3）。这五个基因，nuoJ、nuoK、nuoL、nuoM和nuoN，分别编码与线粒体（mt）亚基ND6、ND4L、ND5、ND4和ND2相关的蛋白质。在Rc中鉴定出的nuo基因的总体组织与反硝化副球菌（Pd）nqo基因簇的组织相似。