血浆中的人巨细胞病毒（CMV）DNA反映了白细胞中CMV DNA的数量。
Human cytomegalovirus (CMV) DNA in plasma reflects quantity of CMV DNA present in leukocytes.
作者信息
Zipeto D, Morris S, Hong C, Dowling A, Wolitz R, Merigan T C, Rasmussen L
机构信息
Division of Infectious Diseases, Stanford University School of Medicine, California 94305, USA.
出版信息
J Clin Microbiol. 1995 Oct;33(10):2607-11. doi: 10.1128/jcm.33.10.2607-2611.1995.
Abstract
A quantitative DNA amplification assay for human cytomegalovirus (CMV) DNA has been used to evaluate the relationship between quantities of CMV DNA in plasma and those in infected leukocytes (WBC) from human immunodeficiency virus-infected patients. The target sequence for DNA amplification was a region of the immediate-early 1 gene of CMV. The quantitation assay uses an internal control that is coamplified with each patient sample DNA and contains a sequence for detection by colorimetric hybridization with the same bases, but in different order than in the CMV immediate-early 1 region used for hybridization of amplified patient sample DNA. Results showed that patients with CMV disease had more CMV DNA in both WBC and plasma than those without disease. However, in this study, copy numbers of CMV DNA in WBC were higher than those in plasma. The gB and gH variants were the same in plasma and WBC.
摘要
一种用于检测人巨细胞病毒（CMV）DNA的定量DNA扩增检测法，已被用于评估血浆中CMV DNA的量与来自人类免疫缺陷病毒感染患者的受感染白细胞（WBC）中CMV DNA的量之间的关系。DNA扩增的靶序列是CMV即刻早期1基因的一个区域。定量检测法使用一种内部对照，该对照与每个患者样本DNA共同扩增，并且包含一个用于通过比色杂交检测的序列，其碱基与用于扩增患者样本DNA杂交的CMV即刻早期1区域相同，但顺序不同。结果显示，患有CMV疾病的患者白细胞和血浆中的CMV DNA均比未患病者更多。然而，在本研究中，白细胞中CMV DNA的拷贝数高于血浆中的拷贝数。血浆和白细胞中的gB和gH变体相同。
相似文献
1
Human cytomegalovirus (CMV) DNA in plasma reflects quantity of CMV DNA present in leukocytes.血浆中的人巨细胞病毒（CMV）DNA反映了白细胞中CMV DNA的数量。
J Clin Microbiol. 1995 Oct;33(10):2607-11. doi: 10.1128/jcm.33.10.2607-2611.1995.
2
Quantitation of human cytomegalovirus DNA from peripheral blood cells of human immunodeficiency virus-infected patients could predict cytomegalovirus retinitis.
J Infect Dis. 1995 Jan;171(1):177-82. doi: 10.1093/infdis/171.1.177.
3
Quantitation of human cytomegalovirus DNA in leukocytes by end-point titration and duplex polymerase chain reaction.通过终点滴定法和双链聚合酶链反应对白细胞中的人巨细胞病毒DNA进行定量分析。
J Virol Methods. 1994 Sep;49(2):195-208. doi: 10.1016/0166-0934(94)90044-2.
4
Human cytomegalovirus DNA is present in CD45+ cells in semen from human immunodeficiency virus-infected patients.人类巨细胞病毒DNA存在于人类免疫缺陷病毒感染患者精液中的CD45+细胞中。
J Infect Dis. 1995 Feb;171(2):432-6. doi: 10.1093/infdis/171.2.432.
5
Cytomegalovirus (CMV) DNA amplification from plasma compared with CMV pp65 antigen (ppUL83) detection in leukocytes for early diagnosis of symptomatic CMV infection in kidney transplant patients.肾移植患者有症状巨细胞病毒（CMV）感染的早期诊断：血浆中CMV DNA扩增与白细胞中CMV pp65抗原（ppUL83）检测的比较
Clin Diagn Virol. 1996 Nov;7(2):99-110. doi: 10.1016/s0928-0197(96)00258-9.
6
Identification of active cytomegalovirus infection by analysis of immediate-early, early and late transcripts in peripheral blood cells of immunodeficient patients.通过分析免疫缺陷患者外周血细胞中的即刻早期、早期和晚期转录本鉴定活动性巨细胞病毒感染。
Mol Cell Probes. 1994 Aug;8(4):261-71. doi: 10.1006/mcpr.1994.1038.
7
Quantification of cytomegalovirus DNA in peripheral blood leukocytes by a branched-DNA signal amplification assay.采用分支DNA信号放大分析法对外周血白细胞中的巨细胞病毒DNA进行定量分析。
J Clin Microbiol. 1997 Nov;35(11):2740-4. doi: 10.1128/jcm.35.11.2740-2744.1997.
8
Detection of CMV DNA in peripheral blood leukocytes as a long-range harbinger of CMV disease in HIV-infected patients. Swiss HIV Cohort Study.检测外周血白细胞中的巨细胞病毒（CMV）DNA作为HIV感染患者CMV疾病的长期预兆。瑞士HIV队列研究。
Ocul Immunol Inflamm. 1999 Dec;7(3-4):147-57. doi: 10.1076/ocii.7.3.147.4001.
9
Single tube competitive PCR for quantitation of CMV DNA in the blood of HIV+ and solid organ transplant patients.用于定量检测HIV阳性患者和实体器官移植患者血液中巨细胞病毒（CMV）DNA的单管竞争性PCR。
J Virol Methods. 1998 Apr;71(2):137-46. doi: 10.1016/s0166-0934(97)00087-6.
10
Detection of ganciclovir resistance mutations quantitation of cytomegalovirus (CMV) DNA in leukocytes of patients with fatal disseminated CMV disease.检测更昔洛韦耐药突变并定量分析致命性播散性巨细胞病毒（CMV）疾病患者白细胞中的CMV DNA。
J Infect Dis. 1996 Mar;173(3):523-8. doi: 10.1093/infdis/173.3.523.
引用本文的文献
1
Peripheral blood leukocytes and serum nested polymerase chain reaction are complementary methods for monitoring active cytomegalovirus infection in transplant patients.外周血白细胞和血清巢式聚合酶链反应是监测移植患者巨细胞病毒感染活动的互补方法。
Can J Infect Dis Med Microbiol. 2013 Fall;24(3):e69-74. doi: 10.1155/2013/214631.
2
Simultaneous quantification of Epstein-Barr virus, cytomegalovirus, and human herpesvirus 6 DNA in samples from transplant recipients by multiplex real-time PCR assay.通过多重实时聚合酶链反应法同时定量检测移植受者样本中的爱泼斯坦-巴尔病毒、巨细胞病毒和人疱疹病毒6型DNA。
J Clin Microbiol. 2007 May;45(5):1426-32. doi: 10.1128/JCM.01515-06. Epub 2007 Mar 21.
3
Comparison of DNA amplification, mRNA amplification, and DNA hybridization techniques for detection of cytomegalovirus in bone marrow transplant recipients.用于检测骨髓移植受者巨细胞病毒的DNA扩增、mRNA扩增及DNA杂交技术的比较
J Clin Microbiol. 2003 Nov;41(11):5159-66. doi: 10.1128/JCM.41.11.5159-5166.2003.
4
Monitoring of cytomegalovirus infection in solid-organ transplant recipients by an ultrasensitive plasma PCR assay.通过超敏血浆PCR检测法监测实体器官移植受者的巨细胞病毒感染
J Clin Microbiol. 2003 Aug;41(8):3757-64. doi: 10.1128/JCM.41.8.3757-3764.2003.
5
Measurement of human cytomegalovirus loads by quantitative real-time PCR for monitoring clinical intervention in transplant recipients.通过定量实时聚合酶链反应检测人巨细胞病毒载量以监测移植受者的临床干预情况。
J Clin Microbiol. 2003 Jan;41(1):187-91. doi: 10.1128/JCM.41.1.187-191.2003.
6
Molecular methods for cytomegalovirus surveillance in bone marrow transplant recipients.骨髓移植受者巨细胞病毒监测的分子方法
J Clin Microbiol. 2002 Nov;40(11):4203-6. doi: 10.1128/JCM.40.11.4203-4206.2002.
7
Human cytomegalovirus DNA in plasma and serum specimens of renal transplant recipients is highly fragmented.肾移植受者血浆和血清样本中的人巨细胞病毒DNA高度碎片化。
J Clin Microbiol. 2002 Nov;40(11):4105-13. doi: 10.1128/JCM.40.11.4105-4113.2002.
8
The genes encoding the gCIII complex of human cytomegalovirus exist in highly diverse combinations in clinical isolates.编码人巨细胞病毒gCIII复合体的基因在临床分离株中以高度多样的组合形式存在。
J Virol. 2002 Nov;76(21):10841-8. doi: 10.1128/jvi.76.21.10841-10848.2002.
9
Multiplex, quantitative, real-time PCR assay for cytomegalovirus and human DNA.用于巨细胞病毒和人类DNA的多重定量实时聚合酶链反应检测法
J Clin Microbiol. 2002 Jul;40(7):2381-6. doi: 10.1128/JCM.40.7.2381-2386.2002.
10
False-positive results of plasma PCR for cytomegalovirus DNA due to delayed sample preparation.由于样本制备延迟导致巨细胞病毒DNA血浆PCR出现假阳性结果。
J Clin Microbiol. 2000 Sep;38(9):3249-53. doi: 10.1128/JCM.38.9.3249-3253.2000.
本文引用的文献
1
Quantification of human cytomegalovirus DNA in peripheral blood polymorphonuclear leukocytes of immunocompromised patients by the polymerase chain reaction.通过聚合酶链反应对免疫功能低下患者外周血多形核白细胞中的人巨细胞病毒DNA进行定量分析。
J Virol Methods. 1993 Sep;44(1):45-55. doi: 10.1016/0166-0934(93)90006-d.
2
Effect of foscarnet induction treatment on quantitation of human cytomegalovirus (HCMV) DNA in peripheral blood polymorphonuclear leukocytes and aqueous humor of AIDS patients with HCMV retinitis. The Italian Foscarnet Study Group.膦甲酸钠诱导治疗对艾滋病合并人巨细胞病毒性视网膜炎患者外周血多形核白细胞和房水中人巨细胞病毒（HCMV）DNA定量的影响。意大利膦甲酸钠研究组。
Antimicrob Agents Chemother. 1994 Jan;38(1):38-44. doi: 10.1128/AAC.38.1.38.
3
Application of PCR to multiple specimen types for diagnosis of cytomegalovirus infection: comparison with cell culture and shell vial assay.聚合酶链反应在多种标本类型诊断巨细胞病毒感染中的应用：与细胞培养和空斑试验的比较
J Clin Microbiol. 1994 Jan;32(1):5-10. doi: 10.1128/jcm.32.1.5-10.1994.
4
Comparison of nested PCR for detection of DNA in plasma with pp65 leukocytic antigenemia procedure for diagnosis of human cytomegalovirus infection.用于检测血浆中DNA的巢式聚合酶链反应与用于诊断人巨细胞病毒感染的pp65白细胞抗原血症检测方法的比较。
J Clin Microbiol. 1994 Jun;32(6):1614-8. doi: 10.1128/jcm.32.6.1614-1618.1994.
5
Detection of cytomegalovirus DNA in sera of liver transplant recipients.肝移植受者血清中巨细胞病毒DNA的检测
J Clin Microbiol. 1994 Jun;32(6):1431-4. doi: 10.1128/jcm.32.6.1431-1434.1994.
6
Comparison of PCR and pp65 antigenemia assay with quantitative shell vial culture for detection of cytomegalovirus in blood leukocytes from solid-organ transplant recipients.比较聚合酶链反应（PCR）、pp65抗原血症检测法与定量空斑试验培养法检测实体器官移植受者血液白细胞中巨细胞病毒的情况。
J Clin Microbiol. 1994 Apr;32(4):997-1003. doi: 10.1128/jcm.32.4.997-1003.1994.
7
Detection of cytomegalovirus DNA in serum correlates with clinical cytomegalovirus retinitis in AIDS.
J Infect Dis. 1994 Nov;170(5):1271-4. doi: 10.1093/infdis/170.5.1271.
8
Comparative quantitation of human cytomegalovirus DNA in blood leukocytes and plasma of transplant and AIDS patients.移植患者和艾滋病患者血液白细胞及血浆中人类巨细胞病毒DNA的定量比较
J Clin Microbiol. 1994 Nov;32(11):2709-17. doi: 10.1128/jcm.32.11.2709-2717.1994.
9
Human cytomegalovirus DNA is present in CD45+ cells in semen from human immunodeficiency virus-infected patients.人类巨细胞病毒DNA存在于人类免疫缺陷病毒感染患者精液中的CD45+细胞中。
J Infect Dis. 1995 Feb;171(2):432-6. doi: 10.1093/infdis/171.2.432.
10
Quantitation of human cytomegalovirus DNA from peripheral blood cells of human immunodeficiency virus-infected patients could predict cytomegalovirus retinitis.
J Infect Dis. 1995 Jan;171(1):177-82. doi: 10.1093/infdis/171.1.177.
Zotero 插件