Zipeto D, Morris S, Hong C, Dowling A, Wolitz R, Merigan T C, Rasmussen L
Division of Infectious Diseases, Stanford University School of Medicine, California 94305, USA.
J Clin Microbiol. 1995 Oct;33(10):2607-11. doi: 10.1128/jcm.33.10.2607-2611.1995.
A quantitative DNA amplification assay for human cytomegalovirus (CMV) DNA has been used to evaluate the relationship between quantities of CMV DNA in plasma and those in infected leukocytes (WBC) from human immunodeficiency virus-infected patients. The target sequence for DNA amplification was a region of the immediate-early 1 gene of CMV. The quantitation assay uses an internal control that is coamplified with each patient sample DNA and contains a sequence for detection by colorimetric hybridization with the same bases, but in different order than in the CMV immediate-early 1 region used for hybridization of amplified patient sample DNA. Results showed that patients with CMV disease had more CMV DNA in both WBC and plasma than those without disease. However, in this study, copy numbers of CMV DNA in WBC were higher than those in plasma. The gB and gH variants were the same in plasma and WBC.
一种用于检测人巨细胞病毒(CMV)DNA的定量DNA扩增检测法,已被用于评估血浆中CMV DNA的量与来自人类免疫缺陷病毒感染患者的受感染白细胞(WBC)中CMV DNA的量之间的关系。DNA扩增的靶序列是CMV即刻早期1基因的一个区域。定量检测法使用一种内部对照,该对照与每个患者样本DNA共同扩增,并且包含一个用于通过比色杂交检测的序列,其碱基与用于扩增患者样本DNA杂交的CMV即刻早期1区域相同,但顺序不同。结果显示,患有CMV疾病的患者白细胞和血浆中的CMV DNA均比未患病者更多。然而,在本研究中,白细胞中CMV DNA的拷贝数高于血浆中的拷贝数。血浆和白细胞中的gB和gH变体相同。