Serologic diagnosis of caprine arthritis-encephalitis by ELISA with two recombinant proteins in a parallel testing format.

A scheme for screening sera for antibodies to caprine arthritis-encephalitis virus (CAEV) was evaluated for its ability to identify positive and negative samples in a population with heterogeneous risk factors, using the criteria of sensitivity, specificity and positive predictive value. Five hundred caprine serum samples were tested using a transmembrane recombinant-based ELISA. Those that gave positive results were considered positive, while those with equivocal or negative results were retested with a core recombinant protein-based ELISA. Equivocal results after the second test were considered indeterminate and retesting is advised. Using this approach, a sensitivity, specificity and positive predictive value of 98.8%, 97.2% and 98.6% were obtained. These values are superior to those obtained by these tests used individually. The high sensitivity, specificity and predictive value of this new scheme of CAEV screening make it an attractive addition to any control or eradication program.