Walsh C M, Hayashi F, Saffran D C, Ju S T, Berke G, Clark W R
Department of Biology, University of California, Los Angeles 90095, USA.
J Immunol. 1996 Feb 15;156(4):1436-41.
Tumor cells insensitive to lysis through the Fas and TNF pathways were injected either subcutaneously or into the peritoneal cavities of allogeneic perforin-less (P0) and perforin wild-type (P2) mice. In three of four cases, the tumors were rejected equally rapidly in both strains of mice. Rejection was accompanied by vigorous in vitro cytotoxicity in P2, but not in P0 mice. The rapid clearance of allografted cells in mice where all three known cytolytic pathways are seriously compromised raises important questions about the involvement of cell-mediated cytotoxicity, as defined by current assay techniques, in primary allograft rejection.
将对通过Fas和TNF途径的细胞溶解不敏感的肿瘤细胞皮下注射或注入同种异体穿孔素缺陷型（P0）和穿孔素野生型（P2）小鼠的腹腔。在四分之三的病例中，两种品系的小鼠体内肿瘤被同等迅速地排斥。P2小鼠的排斥反应伴随着强烈的体外细胞毒性，但P0小鼠没有。在所有三种已知的细胞溶解途径严重受损的小鼠中，同种异体移植细胞的快速清除引发了关于当前检测技术所定义的细胞介导的细胞毒性在原发性同种异体移植排斥反应中的作用的重要问题。
