Kinard G R, Barnett O W, Carner G R
Department of Plant Pathology and Physiology, Clemson University, South Carolina 29634-0377, USA.
J Invertebr Pathol. 1995 Nov;66(3):258-63. doi: 10.1006/jipa.1995.1098.
An insect iridescent virus has been isolated from diseased velvetbean caterpillars, Anticarsia gemmatalis, found in Argentina. The cytopathology is similar to that reported for other iridescent viruses with infected larvae exhibiting blue-purple opalescence. Icosahedral particles (n = 119) purified by sucrose gradient rate zonal centrifugation had dimensions of 145 +/- 7 nm (point-to-point) and 136 +/- 12 nm (side-to-side). The sedimentation coefficient was 2250 +/- 10 S20,w when the virus was suspended in phosphate buffer. Characterization of proteins by SDS-polyacrylamide gel electrophoresis revealed 24 polypeptides with a single major species of 53.6 kDa. Purified viral DNA had a density of 1.6902 g cm-3 in equilibrium ultracentrifugation, corresponding to a guanine:cytosine content of 32.2%. Analysis of digests of this DNA with two restriction enzymes revealed an average molecular size of 181.8 +/- 5.6 kbp. A 499-bp fragment was amplified from the genome of this isolate using the polymerase chain reaction and then sequenced; data suggest this virus is very closely related to IV 1, originally isolated from Tipula paludosa. These properties indicate this virus is an isolate of the genus Iridovirus.
从阿根廷发现的患病绒星毛虫（Anticarsia gemmatalis）中分离出一种昆虫虹彩病毒。其细胞病理学与其他虹彩病毒报道的相似，受感染幼虫呈现蓝紫色珠光。通过蔗糖梯度速率区带离心纯化的二十面体颗粒（n = 119），点对点尺寸为145 +/- 7纳米，边对边尺寸为136 +/- 12纳米。当病毒悬浮在磷酸盐缓冲液中时，沉降系数为2250 +/- 10 S20,w。通过SDS-聚丙烯酰胺凝胶电泳对蛋白质进行表征，发现有24种多肽，其中单一主要种类为53.6 kDa。在平衡超速离心中，纯化的病毒DNA密度为1.6902 g cm-3，对应鸟嘌呤：胞嘧啶含量为32.2%。用两种限制酶对该DNA消化产物进行分析，显示平均分子大小为181.8 +/- 5.6 kbp。使用聚合酶链反应从该分离株的基因组中扩增出一个499 bp的片段，然后进行测序；数据表明该病毒与最初从大蚊（Tipula paludosa）分离出的IV 1密切相关。这些特性表明该病毒是虹彩病毒属的一个分离株。
