Spinola S M, Orazi A, Arno J N, Fortney K, Kotylo P, Chen C Y, Campagnari A A, Hood A F
Department of Medicine, Indiana University, Indianapolis, USA.
J Infect Dis. 1996 Feb;173(2):394-402. doi: 10.1093/infdis/173.2.394.
Human subjects were experimentally infected with Haemophilus ducreyi for up to 2 weeks. Bacterial suspensions were delivered into the epidermis and dermis through puncture wounds made by an allergy-testing device. Subjects developed papular lesions that evolved into pustules resembling natural disease. Some papular lesions resolved spontaneously, indicating that host responses may clear infection. Bacteria were shed intermittently from lesions, suggesting that H. ducreyi may be transmissible before ulceration. Host responses to infection consisted primarily of cutaneous infiltrate of polymorphonuclear leukocytes, Langerhans cells, macrophages, and CD4 T cells of alpha beta lineage. Expression of HLA-DR by keratinocytes was associated with the presence of interferon-gamma mRNA in the skin. There was little evidence for humoral or peripheral blood mononuclear cell responses to bacterial antigens. The cutaneous infiltrate of CD4 cells and macrophages provides a mechanism that facilitates transmission of human immunodeficiency virus by H. ducreyi.
将人类受试者用杜克雷嗜血杆菌进行实验性感染，长达2周。通过过敏测试装置造成的穿刺伤口，将细菌悬液注入表皮和真皮。受试者出现丘疹性病变，这些病变演变成类似自然疾病的脓疱。一些丘疹性病变可自发消退，这表明宿主反应可能清除感染。细菌从病变处间歇性排出，提示杜克雷嗜血杆菌可能在溃疡形成之前具有传染性。宿主对感染的反应主要包括多形核白细胞、朗格汉斯细胞、巨噬细胞和αβ谱系的CD4 T细胞的皮肤浸润。角质形成细胞表达HLA-DR与皮肤中干扰素-γ mRNA的存在相关。几乎没有证据表明对细菌抗原有体液或外周血单核细胞反应。CD4细胞和巨噬细胞的皮肤浸润提供了一种机制，促进杜克雷嗜血杆菌传播人类免疫缺陷病毒。
