Duncan W C, McNeilly A S, Illingworth P J
MRC Reproductive Biology Unit, Centre for Reproductive Biology, Edinburgh, UK.
J Endocrinol. 1996 Jan;148(1):59-67. doi: 10.1677/joe.0.1480059.
Tissue inhibitor of metalloproteinases-1 (TIMP-1) is a specific inhibitor of a group of proteolytic enzymes known as matrix metalloproteinases. These enzymes have been widely implicated in the process of tissue remodelling. Extensive remodelling occurs in the corpus luteum during luteolysis unless human chorionic gonadotrophin (hCG) is produced by the early conceptus. This study aimed to investigate the expression and localisation of TIMP-1 in human corpora lutea during the luteal phase of the cycle and after luteal rescue with exogenous hCG to mimic the changes of early pregnancy. Human corpora lutea from the early (n = 4), mid- (n = 4) and late (n = 4) luteal phases and after luteal rescue by hCG (n = 4) were obtained at the time of hysterectomy. Expression of TIMP-1 was investigated in these tissues by Western blotting, immunohistochemistry, Northern blotting and in situ hybridisation. Luteal cells of thecal origin were distinguished from those of granulosa origin by immunostaining for 17 alpha-hydroxylase. A 30 kDa protein consistent with TIMP-1 was detected in human corpora lutea. This protein was localised to the granulosa lutein cells in all tissues examined. TIMP-1 mRNA was found in large quantities in all glands examined and this again localised to the granulosa lutein cells. The expression and localisation of TIMP-1 did not change throughout the luteal phase and was not altered by luteal rescue. The function of this uniform expression of TIMP-1 in the corpus luteum is not clear but these data suggest that the inhibition of structural luteolysis during maternal recognition of pregnancy is not mediated by regulation of TIMP-1 expression.
金属蛋白酶组织抑制剂-1（TIMP-1）是一组被称为基质金属蛋白酶的蛋白水解酶的特异性抑制剂。这些酶在组织重塑过程中具有广泛作用。在黄体溶解过程中，黄体发生广泛重塑，除非早期胚胎产生人绒毛膜促性腺激素（hCG）。本研究旨在调查TIMP-1在月经周期黄体期及外源性hCG黄体挽救后（以模拟早期妊娠变化）人黄体中的表达及定位。在子宫切除时获取早期（n = 4）、中期（n = 4）和晚期（n = 4）黄体期以及hCG黄体挽救后的人黄体（n = 4）。通过蛋白质免疫印迹法、免疫组织化学、Northern印迹法和原位杂交法研究这些组织中TIMP-1的表达。通过对17α-羟化酶进行免疫染色，将卵泡膜来源的黄体细胞与颗粒细胞来源的黄体细胞区分开来。在人黄体中检测到一种与TIMP-1一致的30 kDa蛋白质。该蛋白质定位于所有检测组织的颗粒黄体细胞中。在所有检测腺体中均发现大量TIMP-1 mRNA，其同样定位于颗粒黄体细胞。TIMP-1的表达及定位在整个黄体期均未改变，且未因黄体挽救而改变。TIMP-1在黄体中这种均匀表达的功能尚不清楚，但这些数据表明，在母体识别妊娠期间对结构性黄体溶解的抑制并非由TIMP-1表达的调节介导。
