Tongiorgi E, Bernhardt R R, Zinn K, Schachner M
Department of Neurobiology, Swiss Federal Institute of Technology, Zürich, Switzerland.
J Neurobiol. 1995 Nov;28(3):391-407. doi: 10.1002/neu.480280311.
A partial zebrafish tenascin-C cDNA clone was isolated from an embryonic zebrafish cDNA library on the basis of homology to mouse tenascin-C. The expression pattern in the head of embryonic zebrafish was analyzed by in situ hybridization. Tenascin-C mRNA was detected in neural crest cells during the period of their migration and differentiation. Expression also occurred in differentiating placodal tissues and in mesodermal cells. In the developing brain, tenascin-C mRNA was expressed in specific domains. In the hindbrain the pattern of the domains was dynamic. At 18 to 22 h postfertilization, expression was widespread in rhombomeres 3, 5, and 6, confined to periventricular cells in rhombomere 2, and not detectable in rhombomere 4. At 32 h postfertilization, tenascin-C was expressed at the rhombomere boundaries. In contrast to the hindbrain, the pattern in the forebrain and midbrain did not show any major changes between 22 and 32 h postfertilization. Domains expressing tenascin-C alternated with regions devoid of it. The most anterior domain of expression was observed at the telencephalic-diencephalic border, surrounding the optic recess. A second domain, at the border between the diencephalon and the midbrain, and a third domain, in the caudal midbrain tegmentum, appeared restricted to the basal plate. Additionally, expression of tenascin-C mRNA was detected in the hypothalamus and in the developing epiphysis. These expression patterns suggest that tenascin-C may play a role in neural crest cell migration and during the differentiation of neural crest, placodal, and mesodermal derivatives. In the developing brain, tenascin-C may be involved in the consolidation of different regional identities.
基于与小鼠腱生蛋白-C的同源性，从斑马鱼胚胎cDNA文库中分离出一个部分斑马鱼腱生蛋白-C cDNA克隆。通过原位杂交分析了胚胎斑马鱼头部的表达模式。在神经嵴细胞迁移和分化期间检测到腱生蛋白-C mRNA。在分化的基板组织和中胚层细胞中也有表达。在发育中的大脑中，腱生蛋白-C mRNA在特定区域表达。在后脑中，这些区域的模式是动态的。在受精后18至22小时，菱脑节3、5和6中广泛表达，局限于菱脑节2的脑室周围细胞，而在菱脑节4中未检测到。在受精后32小时，腱生蛋白-C在菱脑节边界处表达。与后脑不同，前脑和中脑的模式在受精后22至32小时之间没有显示出任何重大变化。表达腱生蛋白-C的区域与没有表达的区域交替出现。观察到最前面的表达区域在端脑-间脑边界，围绕视隐窝。第二个区域在间脑和中脑之间的边界，第三个区域在中脑尾部被盖，似乎局限于基板。此外，在丘脑下部和发育中的松果体中检测到腱生蛋白-C mRNA的表达。这些表达模式表明，腱生蛋白-C可能在神经嵴细胞迁移以及神经嵴、基板和中胚层衍生物的分化过程中发挥作用。在发育中的大脑中，腱生蛋白-C可能参与不同区域特征的巩固。
